Arabidopsis plants overexpressing additional copies of heat shock protein Hsp101 showed high heat tolerance and endo-gene silencing

Arabidopsis plants overexpressing additional copies of heat shock protein Hsp101 showed high heat tolerance and endo-gene silencing

Copyright © 2023 Elsevier B.V. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant science : an international journal of experimental plant biology. - 1985. - 330(2023) vom: 13. Mai, Seite 111639
1. Verfasser: Babbar, Richa (VerfasserIn)
Weitere Verfasser: Tiwari, Lalit Dev, Mishra, Ratnesh Chandra, Shimphrui, Rinchuila, Singh, Aditya Abha, Goyal, Isha, Rana, Surbhi, Kumar, Ritesh, Sharma, Vijyesh, Tripathi, Gayatri, Khungar, Lisha, Sharma, Jaydeep, Agrawal, Chhavi, Singh, Garima, Biswas, Tanya, Biswal, Anup Kumar, Sahi, Chandan, Sarkar, Neelam K, Grover, Anil
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:Plant science : an international journal of experimental plant biology
Schlagworte:Journal Article Arabidopsis AtHsfA2 Choline kinase Gene silencing Heat tolerance Hsp101 Protein aggregates Transgenic lines DNA, Complementary mehr... Heat-Shock Proteins Plant Proteins Transcription Factors
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245 1 0 |a Arabidopsis plants overexpressing additional copies of heat shock protein Hsp101 showed high heat tolerance and endo-gene silencing 
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520 |a Hsp101 chaperone is vital for survival of plants under heat stress. We generated transgenic Arabidopsis thaliana (Arabidopsis) lines with extra copies of Hsp101 gene using diverse approaches. Arabidopsis plants transformed with rice Hsp101 cDNA driven by Arabidopsis Hsp101 promoter (IN lines) showed high heat tolerance while the plants transformed with rice Hsp101 cDNA driven by CaMV35S promoter (C lines) were like wild type plants in heat stress response. Transformation of Col-0 plants with 4633 bp Hsp101 genomic fragment (GF lines) from A. thaliana containing both its coding and the regulatory sequence resulted in mostly over-expressor (OX) lines and a few under-expressor (UX) lines of Hsp101. OX lines showed enhanced heat tolerance while the UX lines were overly heat sensitive. In UX lines, silencing of not only Hsp101 endo-gene was noted but also transcript of choline kinase (CK2) was silenced. Previous work established that in Arabidopsis, CK2 and Hsp101 are convergent gene pairs sharing a bidirectional promoter. The elevated AtHsp101 protein amount in most GF and IN lines was accompanied by lowered CK2 transcript levels under HS. We observed increased methylation of the promoter and gene sequence region in UX lines; however, methylation was lacking in OX lines 
650 4 |a Journal Article 
650 4 |a Arabidopsis 
650 4 |a AtHsfA2 
650 4 |a Choline kinase 
650 4 |a Gene silencing 
650 4 |a Heat tolerance 
650 4 |a Hsp101 
650 4 |a Protein aggregates 
650 4 |a Transgenic lines 
650 7 |a DNA, Complementary  |2 NLM 
650 7 |a Heat-Shock Proteins  |2 NLM 
650 7 |a Plant Proteins  |2 NLM 
650 7 |a Transcription Factors  |2 NLM 
700 1 |a Tiwari, Lalit Dev  |e verfasserin  |4 aut 
700 1 |a Mishra, Ratnesh Chandra  |e verfasserin  |4 aut 
700 1 |a Shimphrui, Rinchuila  |e verfasserin  |4 aut 
700 1 |a Singh, Aditya Abha  |e verfasserin  |4 aut 
700 1 |a Goyal, Isha  |e verfasserin  |4 aut 
700 1 |a Rana, Surbhi  |e verfasserin  |4 aut 
700 1 |a Kumar, Ritesh  |e verfasserin  |4 aut 
700 1 |a Sharma, Vijyesh  |e verfasserin  |4 aut 
700 1 |a Tripathi, Gayatri  |e verfasserin  |4 aut 
700 1 |a Khungar, Lisha  |e verfasserin  |4 aut 
700 1 |a Sharma, Jaydeep  |e verfasserin  |4 aut 
700 1 |a Agrawal, Chhavi  |e verfasserin  |4 aut 
700 1 |a Singh, Garima  |e verfasserin  |4 aut 
700 1 |a Biswas, Tanya  |e verfasserin  |4 aut 
700 1 |a Biswal, Anup Kumar  |e verfasserin  |4 aut 
700 1 |a Sahi, Chandan  |e verfasserin  |4 aut 
700 1 |a Sarkar, Neelam K  |e verfasserin  |4 aut 
700 1 |a Grover, Anil  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Plant science : an international journal of experimental plant biology  |d 1985  |g 330(2023) vom: 13. Mai, Seite 111639  |w (DE-627)NLM098174193  |x 1873-2259  |7 nnns 
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