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20231226054757.0 |
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231226s2023 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2023.02.012
|2 doi
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|a pubmed24n1176.xml
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|a (DE-627)NLM352900784
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|a (NLM)36780721
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|a (PII)S0981-9428(23)00086-4
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Wang, Sheng
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Characterization of Arnebia euchroma PGT homologs involved in the biosynthesis of shikonin
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|c 2023
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 05.04.2023
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|a Date Revised 05.04.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2023 The Authors. Published by Elsevier Masson SAS.. All rights reserved.
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|a Shikonin is a red naphthoquinone natural product from plants with high economical and medical values. The para-hydroxybenzoic acid geranyltransferase (PGT) catalyzes the key regulatory step of shikonin biosynthesis. PGTs from Lithospermum erythrorhizon have been well-characterized and used in industrial shikonin production. However, its perennial medicinal plant Arnebia euchroma accumulates much more pigment and the underlying mechanism remains obscure. Here, we discovered and characterized the different isoforms of AePGTs. Phylogenetic study and structure modeling suggested that the N-terminal of AePGT6 contributed to its highest activity among 7 AePGTs. Indeed, AePGT2 and AePGT3 fused with 60 amino acids from the N-terminal of AePGT6 showed even higher activity than AePGT6, while native AePGT2 and AePGT3 don't have catalytic activity. Our result not only provided a mechanistic explanation of high shikonin contents in Arnebia euchroma but also engineered a best-performing PGT to achieve the highest-to-date production of 3-geranyl-4-hydroxybenzoate acid, an intermedium of shikonin
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|a Journal Article
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|a 3-Geranyl-4-hydroxybenzoate acid
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|a Naphthoquinone
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| 650 |
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4 |
|a Para-hydroxybenzoic acid geranyltransferase
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| 650 |
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4 |
|a Shikonin
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| 650 |
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|a shikonin
|2 NLM
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| 650 |
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|a 3IK6592UBW
|2 NLM
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| 650 |
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|a Naphthoquinones
|2 NLM
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| 650 |
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|a Geranyltranstransferase
|2 NLM
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| 650 |
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7 |
|a EC 2.5.1.10
|2 NLM
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| 700 |
1 |
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|a Shi, Linyuan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Ruishan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, Changzheng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Jinye
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Shen, Ye
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tatsumi, Kanade
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Navrot, Nicolas
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, Tan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Guo, Lanping
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 196(2023) vom: 15. März, Seite 587-595
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
1 |
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|g volume:196
|g year:2023
|g day:15
|g month:03
|g pages:587-595
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| 856 |
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|u http://dx.doi.org/10.1016/j.plaphy.2023.02.012
|3 Volltext
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|a GBV_ILN_350
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|a AR
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|d 196
|j 2023
|b 15
|c 03
|h 587-595
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