miR394 enhances WUSCHEL-induced somatic embryogenesis in Arabidopsis thaliana

miR394 enhances WUSCHEL-induced somatic embryogenesis in Arabidopsis thaliana

© 2023 The Authors New Phytologist © 2023 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 238(2023), 3 vom: 03. Mai, Seite 1059-1072
1. Verfasser: Lu, Lu (VerfasserIn)
Weitere Verfasser: Holt, Anna, Chen, Xinying, Liu, Yang, Knauer, Steffen, Tucker, Elise J, Sarkar, Ananda Kumar, Hao, Zhaodong, Roodbarkelari, Farshad, Shi, Jisen, Chen, Jinhui, Laux, Thomas
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't LEAF CURLING RESPONSIVENESS WUSCHEL ecotype miR394 pluripotency somatic embryogenesis Arabidopsis Proteins Homeodomain Proteins mehr... WUSCHEL protein, Arabidopsis MicroRNAs
Beschreibung
Zusammenfassung:© 2023 The Authors New Phytologist © 2023 New Phytologist Foundation.
Many plant species can give rise to embryos from somatic cells after a simple hormone treatment, illustrating the remarkable developmental plasticity of differentiated plant cells. However, many species are recalcitrant to somatic embryo formation for unknown reasons, which poses a significant challenge to agriculture, where somatic embryogenesis is an important tool to propagate desired genotypes. The micro-RNA394 (miR394) promotes shoot meristem maintenance in Arabidopsis thaliana, but the underlying mechanisms have remained elusive. We analyzed whether miR394 affects indirect somatic embryogenesis and determined the transcriptome of embryogenic callus upon miR394-enhanced somatic embryogenesis. We show that ectopic miR394 expression enhances somatic embryogenesis in the recalcitrant Ler accession when co-expressed with the transcription factor WUSCHEL (WUS) and that miR394 acts in this process through silencing the target LEAF CURLING RESPONSIVENESS (LCR). Furthermore, we show that higher endogenous miR394 levels are required for the elevated embryogenic potential of the Columbia accession compared with Ler, providing a mechanistic explanation for this natural variation. Our transcriptional analysis provides a framework for miR394 function in regulating pluripotency by expanding WUS-mediated direct transcriptional repression
Beschreibung:Date Completed 31.03.2023
Date Revised 13.04.2023
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.18801