A chaperonin containing T-complex polypeptide-1 facilitates the formation of the PbWoxT1-PbPTB3 ribonucleoprotein complex for long-distance RNA trafficking in Pyrus betulaefolia

A chaperonin containing T-complex polypeptide-1 facilitates the formation of the PbWoxT1-PbPTB3 ribonucleoprotein complex for long-distance RNA trafficking in Pyrus betulaefolia

© 2023 The Authors New Phytologist © 2023 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 238(2023), 3 vom: 20. Mai, Seite 1115-1128
1. Verfasser: Wang, Shengnan (VerfasserIn)
Weitere Verfasser: Duan, Xuwei, Wang, Shengyuan, Hao, Li, Zhang, Yi, Xu, Chaoran, Yu, Yunfei, Xiang, Ling, Jiang, Feng, Heinlein, Manfred, Li, Tianzhong, Zhang, Wenna
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't CCT Pyrus betulaefolia RNP complex assembly long-distance trafficking Chaperonin Containing TCP-1 EC 3.6.1.- Plant Proteins mehr... RNA, Messenger RNA, Plant Ribonucleoproteins
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100 1 |a Wang, Shengnan  |e verfasserin  |4 aut 
245 1 2 |a A chaperonin containing T-complex polypeptide-1 facilitates the formation of the PbWoxT1-PbPTB3 ribonucleoprotein complex for long-distance RNA trafficking in Pyrus betulaefolia 
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520 |a © 2023 The Authors New Phytologist © 2023 New Phytologist Foundation. 
520 |a Numerous plant endogenous mRNAs move via phloem and thus affect the growth and development of long-distant organs. mRNAs are transported with RNA-binding proteins forming a ribonucleoprotein complex. However, it remains elusive how such RNP complex assembles and facilitates mRNA trafficking. Protease digestion and RNA immunoprecipitation were used to investigate the RNP assembly function of the complete Chaperonin Containing T-complex Polypeptide-1. In situ hybridization, hairy root transformation, microprojectile bombardment, and grafting experiments demonstrate the role of CCT complex in the transport of a PbWoxT1-PbPTB3 RNP complex in Pyrus betulaefolia. PbCCT5 silenced caused defective movement of GFP-PbPTB3 and GFP-PbWoxT1 from hairy roots to new leaves via the phloem. PbCCT5 is shown to interact with PbPTB3. PbCCT complex enhanced PbPTB3 stabilization and permitted assembly of PbWoxT1 and PbPTB3 into an RNP complex. Furthermore, silencing of individual CCT subunits inhibited the intercellular movement of GFP-PbPTB3 and long-distance trafficking of PbWoxT1 and PbPTB3 in grafted plants. Taken together, the CCT complex assembles PbPTB3 and PbWoxT1 into an RNP complex in the phloem in order to facilitate the long-distance trafficking of PbWoxT1 in P. betulaefolia. This study therefore provides important insights into the mechanism of RNP complex formation and transport 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a CCT 
650 4 |a Pyrus betulaefolia 
650 4 |a RNP complex 
650 4 |a assembly 
650 4 |a long-distance trafficking 
650 7 |a Chaperonin Containing TCP-1  |2 NLM 
650 7 |a EC 3.6.1.-  |2 NLM 
650 7 |a Plant Proteins  |2 NLM 
650 7 |a RNA, Messenger  |2 NLM 
650 7 |a RNA, Plant  |2 NLM 
650 7 |a Ribonucleoproteins  |2 NLM 
700 1 |a Duan, Xuwei  |e verfasserin  |4 aut 
700 1 |a Wang, Shengyuan  |e verfasserin  |4 aut 
700 1 |a Hao, Li  |e verfasserin  |4 aut 
700 1 |a Zhang, Yi  |e verfasserin  |4 aut 
700 1 |a Xu, Chaoran  |e verfasserin  |4 aut 
700 1 |a Yu, Yunfei  |e verfasserin  |4 aut 
700 1 |a Xiang, Ling  |e verfasserin  |4 aut 
700 1 |a Jiang, Feng  |e verfasserin  |4 aut 
700 1 |a Heinlein, Manfred  |e verfasserin  |4 aut 
700 1 |a Li, Tianzhong  |e verfasserin  |4 aut 
700 1 |a Zhang, Wenna  |e verfasserin  |4 aut 
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773 1 8 |g volume:238  |g year:2023  |g number:3  |g day:20  |g month:05  |g pages:1115-1128 
856 4 0 |u http://dx.doi.org/10.1111/nph.18789  |3 Volltext 
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