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231226s2023 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2023.01.034
|2 doi
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|a pubmed25n1174.xml
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|a (DE-627)NLM352485299
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|a (NLM)36738511
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|a (PII)S0981-9428(23)00038-4
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Schmiderer, Corinna
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Monoterpene synthases of three closely related sage species (Salvia officinalis, S. fruticosa and S. pomifera, Lamiaceae)
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| 264 |
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|c 2023
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 05.04.2023
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|a Date Revised 05.04.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2023 The Authors. Published by Elsevier Masson SAS.. All rights reserved.
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|a The diversity of plant monoterpenes is largely based on the catalytic activity of monoterpene synthases. Additionally, copy number variation of monoterpene synthase genes may contribute to the quantity of transcripts and hence to the essential oil profile. This study used whole-genome sequencing and digital PCR for the measurement of copy number variation and quantification of gene expression in three closely related Salvia species, namely Salvia officinalis, Salvia pomifera and Salvia fruticosa. Twelve, 13 and 15 monoterpene synthase-encoding open-reading frames were predicted for Salvia officinalis, Salvia pomifera and Salvia fruticosa, respectively. In Salvia officinalis, one of the open reading frames was disrupted indicating a pseudogene. Monoterpene synthase genes were generally single copy per haploid genome, only a few were double or triple copy genes. Expression levels of monoterpene synthases in leaves corresponded generally well with essential oil composition. In some cases, a higher expression level of a certain monoterpene synthase could be explained by its duplication or triplication. The very high content of thujones in Salvia pomifera, for example, was accompanied by gene duplication and increased gene expression of (+)-sabinene synthase responsible for the thujone precursor sabinene. In Salvia officinalis, three individuals different in their essential oil profile showed significant differences in their monoterpene synthase expression levels corresponding roughly to the profile of the essential oils. Transcript expression of monoterpene synthase genes were measured in leaf, calyx and corolla. The corolla differed significantly from leaves, while calyces usually showed a profile intermediary between leaf and corolla
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| 650 |
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4 |
|a Journal Article
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4 |
|a 1,8-cineole
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| 650 |
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4 |
|a Alpha-thujone
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| 650 |
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|a Beta-thujone
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| 650 |
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4 |
|a Camphor
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| 650 |
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4 |
|a Copy number variation
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| 650 |
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4 |
|a Digital PCR
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| 650 |
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4 |
|a Gene expression
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| 650 |
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4 |
|a Monoterpenes
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| 650 |
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4 |
|a Salvia fruticosa Mill.
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| 650 |
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4 |
|a Salvia officinalis L.
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| 650 |
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4 |
|a Salvia pomifera L.
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| 650 |
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|a sabinene
|2 NLM
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| 650 |
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|a 7D1TL44GPC
|2 NLM
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| 650 |
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|a pinene cyclase I
|2 NLM
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| 650 |
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7 |
|a EC 5.5.-
|2 NLM
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| 650 |
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7 |
|a Monoterpenes
|2 NLM
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| 650 |
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|a Oils, Volatile
|2 NLM
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| 700 |
1 |
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|a Steinborn, Ralf
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Novak, Johannes
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 196(2023) vom: 15. März, Seite 318-327
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnas
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| 773 |
1 |
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|g volume:196
|g year:2023
|g day:15
|g month:03
|g pages:318-327
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| 856 |
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|u http://dx.doi.org/10.1016/j.plaphy.2023.01.034
|3 Volltext
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|d 196
|j 2023
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|h 318-327
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