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231226s2023 xx |||||o 00| ||eng c |
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|a 10.1016/j.plantsci.2023.111627
|2 doi
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|a pubmed25n1174.xml
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|a (DE-627)NLM352470259
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|a (NLM)36737003
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|a (PII)S0168-9452(23)00044-4
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Ishizaki, Takuma
|e verfasserin
|4 aut
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|a In-frame mutation in rice TEOSINTE BRANCHED1 (OsTB1) improves productivity under phosphorus deficiency
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|c 2023
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 31.03.2023
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|a Date Revised 31.03.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.
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|a Tillering is an important trait in rice productivity. We introduced mutations into the coding region of rice TEOSINTE BRANCHED1 (OsTB1), which is a negative regulator of tillering, using CRISPR/Cas9. The frameshift mutants exhibited substantially enhanced tillering and produced 3.5 times more panicles than the non-mutated plants at maturity. This enhanced tillering resulted in increased spikelet number; however, grain yields did not increase due to substantially reduced filled grain rate and 1,000-grain weight. In contrast, in-frame mutations in OsTB1 had the effect of slightly increasing tiller numbers, and the in-frame mutants had 40% more panicles than non-mutated plants. The grain yield of in-frame mutants also did not increase on nutrient-rich soil; however, under phosphorus-deficient conditions, where tillering is constrained, the in-frame mutants gave a significantly higher grain yield than non-mutated plants due to higher spikelet number and maintained filled grain rate. Rice grassy tiller1 (OsGT1)/OsHox12, which is directly regulated by OsTB1 to suppress tillering, was moderately down-regulated in in-frame mutants, suggesting that OsTB1 with the in-frame mutation shows partial function of intact OsTB1 in regulating OsGT1/OsHox12. We propose that mildly enhanced tillering by in-frame mutation of OsTB1 can improve grain yield under low phosphorus conditions
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|a Journal Article
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|a CRISPR/Cas9
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|a In-frame mutation
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|a OsTB1
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|a Phosphorus deficiency
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|a Rice
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|a TEOSINTE BRANCHED1
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| 650 |
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|a Phosphorus
|2 NLM
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| 650 |
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|a 27YLU75U4W
|2 NLM
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| 650 |
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|a Plant Proteins
|2 NLM
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| 700 |
1 |
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|a Ueda, Yoshiaki
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Takai, Toshiyuki
|e verfasserin
|4 aut
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| 700 |
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|a Maruyama, Kyonoshin
|e verfasserin
|4 aut
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| 700 |
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|a Tsujimoto, Yasuhiro
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 330(2023) vom: 15. Mai, Seite 111627
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnas
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| 773 |
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|g volume:330
|g year:2023
|g day:15
|g month:05
|g pages:111627
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|u http://dx.doi.org/10.1016/j.plantsci.2023.111627
|3 Volltext
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