|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM35199372X |
| 003 |
DE-627 |
| 005 |
20231226052717.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231226s2023 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1021/acs.langmuir.2c02682
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n1173.xml
|
| 035 |
|
|
|a (DE-627)NLM35199372X
|
| 035 |
|
|
|a (NLM)36688667
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Clark, Rose A
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Tripeptide Self-Assembled Monolayers as Biocompatible Surfaces for Cytochrome c Electrochemistry
|
| 264 |
|
1 |
|c 2023
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 01.02.2023
|
| 500 |
|
|
|a Date Revised 27.02.2023
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Biocompatible tripeptide self-assembled monolayers (SAMs) are designed with a carboxylate group on the terminal amino acid (glutamate, aspartate, or amino adipate) to electrostatically attract the lysine groups around the heme crevice in horse heart cytochrome c (cyt c), creating an electroactive protein/tripeptide/Au interfacial structure. Exposing the peptide/Au electrode to cyt c resulted in an 11 ± 3 pmol/cm2 electroactive protein surface coverage. Topographical images of the interfacial structure are obtained down to single-protein resolution by atomic force microscopy. Uniform protein monolayer assemblies are formed on the Au electrode with no major surface roughness changes. The cyt c/peptide/Au electrode systems were examined electrochemically to probe surface charge effects on the redox thermodynamics and kinetics of cyt c. Neutralization of protein surface charge due to adsorption on anionic COOH-terminated SAMs was found to change the formal potential, as determined by cyclic voltammetry. The cyt c/peptide/Au electrodes exhibit formal potentials shifted to more positive values, have a surface carboxylic acid pKa of 6 or higher, and produce effective cyt c surface charges (Zox) of -6 to -14. The Marcus theory is utilized to determine the protein electron transfer rates, which are ∼5 times faster for cyt c/tripeptide/Au compared to cyt c/11-mercaptoundecanoic acid SAMs of similar chain lengths
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
4 |
|a Research Support, U.S. Gov't, Non-P.H.S.
|
| 650 |
|
7 |
|a Cytochromes c
|2 NLM
|
| 650 |
|
7 |
|a 9007-43-6
|2 NLM
|
| 650 |
|
7 |
|a Proteins
|2 NLM
|
| 650 |
|
7 |
|a Peptides
|2 NLM
|
| 650 |
|
7 |
|a Gold
|2 NLM
|
| 650 |
|
7 |
|a 7440-57-5
|2 NLM
|
| 700 |
1 |
|
|a Yawitz, Tanner
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Luchs, Logan
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Conrad, Tiffany
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Bartlebaugh, Owen
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Boyd, Hannah
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Hargittai, Balazs
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1999
|g 39(2023), 4 vom: 31. Jan., Seite 1414-1424
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
|
| 773 |
1 |
8 |
|g volume:39
|g year:2023
|g number:4
|g day:31
|g month:01
|g pages:1414-1424
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1021/acs.langmuir.2c02682
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_22
|
| 912 |
|
|
|a GBV_ILN_350
|
| 912 |
|
|
|a GBV_ILN_721
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 39
|j 2023
|e 4
|b 31
|c 01
|h 1414-1424
|