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|a 10.1093/jxb/erad028
|2 doi
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|a pubmed24n1172.xml
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|a (NLM)36656734
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Sun, Wan-Ting
|e verfasserin
|4 aut
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|a Sugars and sucrose transporters in pollinia of Phalaenopsis aphrodite (Orchidaceae)
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|c 2023
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 20.04.2023
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|a Date Revised 04.06.2023
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|a published: Print
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|a Citation Status MEDLINE
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|a © The Author(s) 2023. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.
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|a The pollen grains of Phalaenopsis orchids are clumped tightly together, packed in pollen dispersal units called pollinia. In this study, the morphology, cytology, biochemistry, and sucrose transporters in pollinia of Phalaenopsis orchids were investigated. Histochemical detection was used to characterize the distribution of sugars and callose at the different development stages of pollinia. Ultra-performance liquid chromatography-high resolution-tandem mass spectrometry data indicated that P. aphrodite accumulated abundant saccharides such as sucrose, galactinol, myo-inositol, and glucose, and trace amounts of raffinose and trehalose in mature pollinia. We found that galactinol synthase (PAXXG304680) and trehalose-6-phosphate phosphatase (PAXXG016120) genes were preferentially expressed in mature pollinia. The P. aphrodite genome was identified as having 11 sucrose transporters (SUTs). Our qRT-PCR confirmed that two SUTs (PAXXG030250 and PAXXG195390) were preferentially expressed in the pollinia. Pollinia germinated in pollen germination media (PGM) supplemented with 10% sucrose showed increased callose production and enhanced pollinia germination, but there was no callose or germination in PGM without sucrose. We show that P. aphrodite accumulates high levels of sugars in mature pollinia, providing nutrients and enhanced SUT gene expression for pollinia germination and tube growth
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Orchidaceae
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|a UPLC-HR-MS/MS
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|a pollen germination
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|a pollinia
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|a saccharides
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|a sucrose transporter
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|a sugar biosynthesis
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|a Sugars
|2 NLM
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|a Sucrose
|2 NLM
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|a 57-50-1
|2 NLM
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| 650 |
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|a Membrane Transport Proteins
|2 NLM
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| 700 |
1 |
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|a Cheng, Sy-Chyi
|e verfasserin
|4 aut
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|a Chao, Ya-Ting
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lin, Shu-Yao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yang, Ting-Ting
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ho, Yi-Ping
|e verfasserin
|4 aut
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| 700 |
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|a Shih, Ming-Che
|e verfasserin
|4 aut
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| 700 |
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|a Ko, Swee-Suak
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 74(2023), 8 vom: 18. Apr., Seite 2556-2571
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:74
|g year:2023
|g number:8
|g day:18
|g month:04
|g pages:2556-2571
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| 856 |
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|u http://dx.doi.org/10.1093/jxb/erad028
|3 Volltext
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