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231226s2023 xx |||||o 00| ||eng c |
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|a 10.1111/nph.18728
|2 doi
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|a pubmed24n1171.xml
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|a (DE-627)NLM351429514
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|a (NLM)36631978
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Méndez-López, Eduardo
|e verfasserin
|4 aut
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| 245 |
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|a Tomato SlGSTU38 interacts with the PepMV coat protein and promotes viral infection
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|c 2023
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 06.03.2023
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|a Date Revised 12.04.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2023 The Authors. New Phytologist © 2023 New Phytologist Foundation.
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|a Pepino mosaic virus (PepMV) is pandemic in tomato crops, causing important economic losses world-wide. No PepMV-resistant varieties have been developed yet. Identification of host factors interacting with PepMV proteins is a promising source of genetic targets to develop PepMV-resistant varieties. The interaction between the PepMV coat protein (CP) and the tomato glutathione S-transferase (GST) SlGSTU38 was identified in a yeast two-hybrid (Y2H) screening and validated by directed Y2H and co-immunoprecipitation assays. SlGSTU38-knocked-out Micro-Tom plants (gstu38) generated by the CRISPR/Cas9 technology together with live-cell imaging were used to understand the role of SlGSTU38 during infection. The transcriptomes of healthy and PepMV-infected wild-type (WT) and gstu38 plants were profiled by RNA-seq analysis. SlGSTU38 functions as a PepMV-specific susceptibility factor in a cell-autonomous manner and relocalizes to the virus replication complexes during infection. Besides, knocking out SlGSTU38 triggers reactive oxygen species accumulation in leaves and the deregulation of stress-responsive genes. SlGSTU38 may play a dual role: On the one hand, SlGSTU38 may exert a proviral function depending on its specific interaction with the PepMV CP; and on the other hand, SlGSTU38 may delay PepMV-infection sensing by participating in the redox intracellular homeostasis in a nonspecific manner
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a PepMV
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|a host factor
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|a potexvirus
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| 650 |
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|a redox homeostasis
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| 650 |
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|a resistance
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|a stress response
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|a viral replication complex (VRC)
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1 |
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|a Donaire, Livia
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gosálvez, Blanca
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Díaz-Vivancos, Pedro
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sánchez-Pina, M Amelia
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tilsner, Jens
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Aranda, Miguel A
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t The New phytologist
|d 1979
|g 238(2023), 1 vom: 15. Apr., Seite 332-348
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
1 |
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|g volume:238
|g year:2023
|g number:1
|g day:15
|g month:04
|g pages:332-348
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| 856 |
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|u http://dx.doi.org/10.1111/nph.18728
|3 Volltext
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|d 238
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|c 04
|h 332-348
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