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231226s2023 xx |||||o 00| ||eng c |
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|a 10.1111/nph.18724
|2 doi
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|a pubmed24n1171.xml
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|a (DE-627)NLM351387455
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|a (NLM)36627730
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Markusch, Hanna
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Elongation factor 1 is a component of the Arabidopsis RNA polymerase II elongation complex and associates with a subset of transcribed genes
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|c 2023
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 09.03.2023
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|a Date Revised 12.04.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2023 The Authors. New Phytologist © 2023 New Phytologist Foundation.
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|a Elongation factors modulate the efficiency of mRNA synthesis by RNA polymerase II (RNAPII) in the context of chromatin, thus contributing to implement proper gene expression programmes. The zinc-finger protein elongation factor 1 (ELF1) is a conserved transcript elongation factor (TEF), whose molecular function so far has not been studied in plants. Using biochemical approaches, we examined the interaction of Arabidopsis ELF1 with DNA and histones in vitro and with the RNAPII elongation complex in vivo. In addition, cytological assays demonstrated the nuclear localisation of the protein, and by means of double-mutant analyses, interplay with genes encoding other elongation factors was explored. The genome-wide distribution of ELF1 was addressed by chromatin immunoprecipitation. ELF1 isolated from Arabidopsis cells robustly copurified with RNAPII and various other elongation factors including SPT4-SPT5, SPT6, IWS1, FACT and PAF1C. Analysis of a CRISPR-Cas9-mediated gene editing mutant of ELF1 revealed distinct genetic interactions with mutants deficient in other elongation factors. Moreover, ELF1 associated with genomic regions actively transcribed by RNAPII. However, ELF1 occupied only c. 33% of the RNAPII transcribed loci with preference for inducible rather than constitutively expressed genes. Collectively, these results establish that Arabidopsis ELF1 shares several characteristic attributes with RNAPII TEFs
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Arabidopsis thaliana
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|a RNA polymerase II
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|a chromatin
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|a gene activity
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| 650 |
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|a mRNA synthesis
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| 650 |
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|a transcript elongation
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| 650 |
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|a Arabidopsis Proteins
|2 NLM
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| 650 |
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|a RNA Polymerase II
|2 NLM
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| 650 |
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|a EC 2.7.7.-
|2 NLM
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| 650 |
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|a SPT4 protein, Arabidopsis
|2 NLM
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| 650 |
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|a Transcriptional Elongation Factors
|2 NLM
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| 700 |
1 |
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|a Michl-Holzinger, Philipp
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Obermeyer, Simon
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Thorbecke, Claudia
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bruckmann, Astrid
|e verfasserin
|4 aut
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| 700 |
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|a Babl, Sabrina
|e verfasserin
|4 aut
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| 700 |
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|a Längst, Gernot
|e verfasserin
|4 aut
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| 700 |
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|a Osakabe, Akihisa
|e verfasserin
|4 aut
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| 700 |
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|a Berger, Frédéric
|e verfasserin
|4 aut
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| 700 |
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|a Grasser, Klaus D
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t The New phytologist
|d 1979
|g 238(2023), 1 vom: 20. Apr., Seite 113-124
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
1 |
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|g volume:238
|g year:2023
|g number:1
|g day:20
|g month:04
|g pages:113-124
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|u http://dx.doi.org/10.1111/nph.18724
|3 Volltext
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|d 238
|j 2023
|e 1
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|c 04
|h 113-124
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