Amberlite XAD-4 is a convenient tool for removing Triton X-100 and Sarkosyl from protein solutions
Amberlite has been shown to be an appropriate material for the adsorption of organic contaminants from aqueous solutions. In addition, Amberlite XAD-2 has been successfully used, as an alternative to Bio-Beads, to remove Triton X-100 from protein solutions, such as from samples of solubilized membra...
| Veröffentlicht in: | BioTechniques. - 1988. - 74(2023), 1 vom: 27. Jan., Seite 45-50 |
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| Weitere Verfasser: | , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2023
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Amberlite XAD-2 Amberlite XAD-4 CHAPS Escherichia coli N-lauroylsarcosine Triton X-100 inclusion bodies amberlite 9079-25-8 mehr... |
| Zusammenfassung: | Amberlite has been shown to be an appropriate material for the adsorption of organic contaminants from aqueous solutions. In addition, Amberlite XAD-2 has been successfully used, as an alternative to Bio-Beads, to remove Triton X-100 from protein solutions, such as from samples of solubilized membrane proteins. However, Amberlite has not been tested as an adsorbent when a mixture of detergents is necessary to solubilize and refold a target protein. Here the authors show that Amberlite XAD-4 can be appropriately used to aid the purification process of proteins solubilized from inclusion bodies with the ternary detergent system consisting of Sarkosyl, Triton X-100 and CHAPS |
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| Beschreibung: | Date Completed 30.01.2023 Date Revised 22.03.2023 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1940-9818 |
| DOI: | 10.2144/btn-2022-0098 |