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231226s2023 xx |||||o 00| ||eng c |
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|a 10.1016/j.plantsci.2022.111578
|2 doi
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|a pubmed24n1170.xml
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|a (DE-627)NLM351199780
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|a (NLM)36608875
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|a (PII)S0168-9452(22)00403-4
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Chen, Yanan
|e verfasserin
|4 aut
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| 245 |
1 |
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|a SlERF.J2 reduces chlorophyll accumulation and inhibits chloroplast biogenesis and development in tomato leaves
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| 264 |
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|c 2023
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 07.02.2023
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|a Date Revised 07.02.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2023 Elsevier B.V. All rights reserved.
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|a Chlorophyll metabolism and chloroplast biogenesis in tomato (Solanum lycopersicum) leaves contribute to photosynthesis; however, their molecular mechanisms are poorly understood. In this study, we found that overexpression of SlERF.J2 (ethylene transcription factor) resulted in a decrease in leaf chlorophyll content and reduced accumulation of starch and soluble sugar. The slerf.j2 knockout mutant showed no apparent change. Further observation of tissue sections and transmission electron microscopy (TEM) showed that SlERF.J2 was involved in chlorophyll accumulation and chloroplast formation. RNA-seq of mature SlERF.J2-OE leaves showed that many genes involved in chlorophyll biosynthesis and chloroplast formation were significantly downregulated compared with those in WT leaves. Genome global scanning of the ERF TF binding site combined with RNA-seq differential gene expression and qRT-PCR detection analysis showed that COP1 was a potential target gene of SlERF.J2. Tobacco transient expression technology, a dual-luciferase reporter system and Y1H technology were employed to verify that SlERF.J2 could bind to the COP1 promoter. Notably, overexpression of SlERF.J2 in Nr mutants resulted in impaired chloroplast biogenesis and development. Taken together, our findings demonstrated that SlERF.J2 plays an essential role in chlorophyll accumulation and chloroplast formation, laying a foundation for enhancing plant photosynthesis
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|a Journal Article
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|a CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1)
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|a Chlorophyll
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|a Chloroplast development
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|a Nr mutants
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| 650 |
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|a SlERF.J2
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| 650 |
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|a Tomato
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| 650 |
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|a Chlorophyll
|2 NLM
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| 650 |
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|a 1406-65-1
|2 NLM
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| 650 |
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|a Plant Proteins
|2 NLM
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| 700 |
1 |
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|a Cai, Xi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tang, Boyan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xie, Qiaoli
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Guoping
|e verfasserin
|4 aut
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| 700 |
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|a Chen, Xuqing
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hu, Zongli
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 328(2023) vom: 10. März, Seite 111578
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnns
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| 773 |
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|g volume:328
|g year:2023
|g day:10
|g month:03
|g pages:111578
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|u http://dx.doi.org/10.1016/j.plantsci.2022.111578
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