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|a 10.1111/jfd.13751
|2 doi
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|a DE-627
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|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Nguyen, Phuong
|e verfasserin
|4 aut
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| 245 |
1 |
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|a An epidemiological analysis of Nocardia seriolae isolated from a wide range of aquatic animals in Taiwan, based on their genotype and enzymatic activity
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|c 2023
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 10.03.2023
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|a Date Revised 10.03.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2023 John Wiley & Sons Ltd.
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|a Chronic disease following Nocardia seriolae infection in a wide range of aquatic animals has been reported in many Asian countries and recently in America and Mexico. This study aimed to investigate the epidemiological relationship among N. seriolae isolates in Taiwan by investigating their genotype and enzymatic activities. A total of 66 strains isolated from 14 known and four unknown host fish from five sites in Taiwan were characterized using five combined methods. High genotypic diversity was recognized among the isolates with 10 pulsotypes being identified from the pulsed-field gel electrophoresis method and 21 reptypes from the repetitive extragenic palindromic amplification method; however, no natural plasmids were detected in this bacterial population. Pulsotypes A8 and RI analysed by PFGE and repPCR, respectively, were found to be predominant within five sites in Taiwan over 17 years of isolation. Enzymatically, the majority of isolates displayed high leucine arylamidase, β-glucosidase and α-glucosidase activities but were negative for lipase, α-galactosidase, β-glucuronidase, N-acetyl-glucosaminidase, α-mannosidase and α-fucosidase activities. We identified a strong association between genotype and enzymatic activity since the majority of pulsotypes displayed the same type of enzymatic profile. This study provides comprehensive and potential epidemiological data, which will aid the fish farming activities and prevention method development
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|a Journal Article
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|a Nocardia seriolae
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| 650 |
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4 |
|a PFGE
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| 650 |
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|a enzymatic profiles
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| 650 |
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4 |
|a genotype
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| 650 |
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4 |
|a repPCR
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| 700 |
1 |
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|a Nazareth, Sandra Celenia
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Mei-Yun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Pei-Chi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Shih-Chu
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of fish diseases
|d 1998
|g 46(2023), 4 vom: 06. Apr., Seite 381-394
|w (DE-627)NLM098166034
|x 1365-2761
|7 nnns
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| 773 |
1 |
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|g volume:46
|g year:2023
|g number:4
|g day:06
|g month:04
|g pages:381-394
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| 856 |
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|u http://dx.doi.org/10.1111/jfd.13751
|3 Volltext
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