Prediction of high fecal propionate-to-butyrate ratios using 16S rRNA-based detection of bacterial groups with liquid array diagnostics

Prediction of high fecal propionate-to-butyrate ratios using 16S rRNA-based detection of bacterial groups with liquid array diagnostics

Butyrate and propionate represent two of three main short-chain fatty acids produced by the intestinal microbiota. In healthy populations, their levels are reportedly equimolar, whereas a deviation in their ratio has been observed in various diseased cohorts. Monitoring such a ratio represents a val...

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Veröffentlicht in:BioTechniques. - 1988. - 74(2023), 1 vom: 18. Jan., Seite 9-21
1. Verfasser: Hiseni, Pranvera (VerfasserIn)
Weitere Verfasser: Snipen, Lars, Wilson, Robert C, Furu, Kari, Hegge, Finn Terje, Rudi, Knut
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't LAD SCFA butyrate gut microbiome propionate qPCR Butyrates Propionates mehr... RNA, Ribosomal, 16S Fatty Acids, Volatile
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520 |a Butyrate and propionate represent two of three main short-chain fatty acids produced by the intestinal microbiota. In healthy populations, their levels are reportedly equimolar, whereas a deviation in their ratio has been observed in various diseased cohorts. Monitoring such a ratio represents a valuable metric; however, it remains a challenge to adopt short-chain fatty acid detection techniques in clinical settings because of the volatile nature of these acids. Here we aimed to estimate short-chain fatty acid information indirectly through a novel, simple quantitative PCR-compatible assay (liquid array diagnostics) targeting a limited number of microbiome 16S markers. Utilizing 15 liquid array diagnostics probes to target microbiome markers selected by a model that combines partial least squares and linear discriminant analysis, the classes (normal vs high propionate-to-butyrate ratio) separated at a threshold of 2.6 with a prediction accuracy of 96% 
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700 1 |a Hegge, Finn Terje  |e verfasserin  |4 aut 
700 1 |a Rudi, Knut  |e verfasserin  |4 aut 
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