Immobilized enzyme with sustainable chestnut biochar to remediate polycyclic aromatic hydrocarbons contaminated soils

Polycyclic aromatic hydrocarbons (PAHs) contaminated soil severely and are difficult to remediate. In this study, acid-modified chestnut inner shell biochar with abundant pore channels was used as the main raw materials for the immobilization of white-rot fungal crude enzyme. The maximum immobilizat...

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Veröffentlicht in:Environmental technology. - 1993. - 45(2024), 10 vom: 05. März, Seite 2034-2044
1. Verfasser: Zhao, Huan (VerfasserIn)
Weitere Verfasser: Wang, Litao, Bai, Yuhong, Li, Ying, Tang, Teng, Liang, Hong, Gao, Dawen
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:Environmental technology
Schlagworte:Journal Article White rot fungus benzo[a]pyrene chestnut-based biochar immobilized crude enzymes polycyclic aromatic hydrocarbons Polycyclic Aromatic Hydrocarbons Soil Enzymes, Immobilized biochar mehr... Charcoal 16291-96-6 Soil Pollutants
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520 |a Polycyclic aromatic hydrocarbons (PAHs) contaminated soil severely and are difficult to remediate. In this study, acid-modified chestnut inner shell biochar with abundant pore channels was used as the main raw materials for the immobilization of white-rot fungal crude enzyme. The maximum immobilization rate of crude enzymes (97.25%±6.20%) could be achieved under the optimal conditions of 24 h immobilization of 10 U/mL crude enzymes by 1 g biochar at 25℃ and pH = 5. Meanwhile, immobilization improved the stability of the crude enzyme. The relative activity of the immobilized crude enzyme increased by 59.32% and 49.73% (compared to the free crude enzymes) after 5 weeks of storage at 4°C and 25°C, respectively. It has been verified that chestnut-based immobilized crude enzyme can degrade 37% of benzo[a]pyrene in 10 days for PAHs-contaminated soils. An efficient, feasible, and low-cost remediation method for PAHs-contaminated soils was explored, which provides technical support for the application of crude enzymes in organic contaminated soils 
650 4 |a Journal Article 
650 4 |a White rot fungus 
650 4 |a benzo[a]pyrene 
650 4 |a chestnut-based biochar 
650 4 |a immobilized crude enzymes 
650 4 |a polycyclic aromatic hydrocarbons 
650 7 |a Polycyclic Aromatic Hydrocarbons  |2 NLM 
650 7 |a Soil  |2 NLM 
650 7 |a Enzymes, Immobilized  |2 NLM 
650 7 |a biochar  |2 NLM 
650 7 |a Charcoal  |2 NLM 
650 7 |a 16291-96-6  |2 NLM 
650 7 |a Soil Pollutants  |2 NLM 
700 1 |a Wang, Litao  |e verfasserin  |4 aut 
700 1 |a Bai, Yuhong  |e verfasserin  |4 aut 
700 1 |a Li, Ying  |e verfasserin  |4 aut 
700 1 |a Tang, Teng  |e verfasserin  |4 aut 
700 1 |a Liang, Hong  |e verfasserin  |4 aut 
700 1 |a Gao, Dawen  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Environmental technology  |d 1993  |g 45(2024), 10 vom: 05. März, Seite 2034-2044  |w (DE-627)NLM098202545  |x 1479-487X  |7 nnns 
773 1 8 |g volume:45  |g year:2024  |g number:10  |g day:05  |g month:03  |g pages:2034-2044 
856 4 0 |u http://dx.doi.org/10.1080/09593330.2022.2164221  |3 Volltext 
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