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01000naa a22002652 4500 |
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NLM350604517 |
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DE-627 |
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20231226045355.0 |
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cr uuu---uuuuu |
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231226s2023 xx |||||o 00| ||eng c |
| 024 |
7 |
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|a 10.1094/PDIS-10-22-2431-RE
|2 doi
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| 028 |
5 |
2 |
|a pubmed24n1168.xml
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|a (DE-627)NLM350604517
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|a (NLM)36548922
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Hodson, Amanda K
|e verfasserin
|4 aut
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| 245 |
1 |
2 |
|a A Real-Time PCR Assay to Detect and Quantify Root-Knot Nematodes from Soil Extracts
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| 264 |
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1 |
|c 2023
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 27.07.2023
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| 500 |
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|a Date Revised 27.07.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Root-knot nematodes cause forking and stubbing of the growing carrot root tip, decreasing market value and reducing yield by up to 45%. Since crop damage by these nematodes depends on their initial population densities at planting, preplant detection of potentially low nematode numbers is critical for predicting future yield loss. The aim of this study was to overcome some of the drawbacks of the labor- and time-intensive process of root-knot nematode identification and quantification by developing and field testing a real-time PCR (qPCR) assay. Primers were designed targeting the root-knot nematode Meloidogyne incognita species complex, which includes M. incognita as well as the closely related Meloidogyne javanica and Meloidogyne arenaria. The qPCR assay successfully detected each species and showed little amplification for nontarget nematode groups except for the sister group Meloidogyne enterolobii, which is not known to occur in California. Predicted nematode densities related well with microscopic counts of nematodes from prepared solutions, as well as from solutions extracted from field soil. In a greenhouse experiment, the qPCR assay distinguished between low, medium, and high levels of M. incognita infection and qPCR predicted densities at planting were negatively related in linear models with final carrot fresh weight, length, and diameter. These results suggest that qPCR assays could be a valuable diagnostic tool to predict nematode infections and prevent crop losses.[Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a Meloidogyne arenaria
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| 650 |
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4 |
|a Meloidogyne incognita
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| 650 |
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4 |
|a Meloidogyne javanica
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| 650 |
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4 |
|a carrot
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| 650 |
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4 |
|a molecular diagnostic
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| 650 |
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4 |
|a nematodes
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| 650 |
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4 |
|a pathogen detection
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| 650 |
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4 |
|a plant-parasitic nematode
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| 650 |
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4 |
|a qPCR
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| 650 |
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4 |
|a vegetables
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| 650 |
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7 |
|a DNA Primers
|2 NLM
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| 650 |
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7 |
|a Soil
|2 NLM
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| 700 |
1 |
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|a Celayir, Tugce
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Quiroz Alonso, Alejandra
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Plant disease
|d 1997
|g 107(2023), 7 vom: 22. Juli, Seite 2169-2176
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnns
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| 773 |
1 |
8 |
|g volume:107
|g year:2023
|g number:7
|g day:22
|g month:07
|g pages:2169-2176
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| 856 |
4 |
0 |
|u http://dx.doi.org/10.1094/PDIS-10-22-2431-RE
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