Physicochemical and bioinformatic characterization of Oreochromis niloticus vitellogenin as an endocrine disruption biomarker
© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
| Veröffentlicht in: | Ecotoxicology (London, England). - 1992. - 32(2023), 1 vom: 22. Jan., Seite 12-24 |
|---|---|
| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2023
|
| Zugriff auf das übergeordnete Werk: | Ecotoxicology (London, England) |
| Schlagworte: | Journal Article 3D model Bioassay Ecotoxicology Immunoassay Nile tilapia Phylogenetic Biomarkers Ethinyl Estradiol 423D2T571U mehr... |
| Zusammenfassung: | © 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. Aquatic biota is increasingly being exposed to chemical pollutants due to human activities and the relationship between the level of environmental pollution and fish reproduction is a continuously ongoing issue. The vitellogenin (Vtg) protein synthesis can be induced in the liver of juvenile and male fish after stimulation of the estrogen receptor and therefore, Vtg has been used as a biomarker of xenoestrogen exposure in several fish species. The current study reported the first physicochemical characterization of Vtg from Oreochromis niloticus. Adult male fish were exposed to 17α-ethinylestradiol for Vtg induction. Purified vitellogenin from plasma showed low stability at 25 and 4 °C in saline conditions, and good stability in acidic (low pH) or in heated conditions. The 3D modeling provided useful information on the structure of O. niloticus Vtg showing conserved structural features. According to bioinformatics and experimental results, there are important structural differences between the two chemical forms of Vtg (VtgAb and VtgC) in a phylogenetic context. The present results add information about the development of ecotoxicological immunoassays to study the endocrine disruption in O. niloticus improving the Vtg performance as a biomarker of reproduction in fish |
|---|---|
| Beschreibung: | Date Completed 07.02.2023 Date Revised 07.02.2023 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1573-3017 |
| DOI: | 10.1007/s10646-022-02612-9 |