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|a 10.1093/jxb/erac499
|2 doi
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|a eng
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|a Dourmap, Corentin
|e verfasserin
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|a Carbon and nitrogen remobilization during seed filling in Arabidopsis is strongly impaired in the pyrroline-5-carboxylate dehydrogenase mutant
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|c 2023
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 16.03.2023
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|a Date Revised 04.04.2023
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|a published: Print
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|a Citation Status MEDLINE
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|a © The Author(s) 2022. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.
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|a Proline is an amino acid that is degraded in the mitochondria by the sequential action of proline dehydrogenase (ProDH) and pyrroline-5-carboxylate dehydrogenase (P5CDH) to form glutamate. We investigated the phenotypes of Arabidopsis wild-type plants, the knockout prodh1 prodh2 double-mutant, and knockout p5cdh allelic mutants grown at low and high nitrate supplies. Surprisingly, only p5cdh presented lower seed yield and produced lighter seeds. Analyses of elements in above-ground organs revealed lower C concentrations in the p5cdh seeds. Determination of C, N, and dry matter partitioning among the above-ground organs revealed a major defect in stem-to-seed resource allocations in this mutant. Again surprisingly, defects in C, N, and biomass allocation to seeds dramatically increased in high-N conditions. 15N-labelling consistently confirmed the defect in N remobilization from the rosette and stem to seeds in p5cdh. Consequently, the p5cdh mutants produced morphologically abnormal, C-depleted seeds that displayed very low germination rates. The most striking result was the strong amplification of the N-remobilization defects in p5cdh under high nitrate supply, and interestingly this phenotype was not observed in the prodh1 prodh2 double-mutant irrespective of nitrate supply. This study reveals an essential role of P5CDH in carbon and nitrogen remobilization for reserve accumulation during seed development in Arabidopsis
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Arabidopsis thaliana
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|a carbon and nitrogen remobilization
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|a proline catabolism
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|a pyrroline-5-carboxylate dehydrogenase
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|a reserve accumulation
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|a seed development
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|a Arabidopsis Proteins
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|a Carbon
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|a 7440-44-0
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|a delta-1-pyrroline-5-carboxylate
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|a 2906-39-0
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|a Nitrates
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|a Nitrogen
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|a N762921K75
|2 NLM
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|a Proline Oxidase
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|a EC 1.5.3.-
|2 NLM
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|a ALDH12A1 protein, Arabidopsis
|2 NLM
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|a EC 1.2.1.88
|2 NLM
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| 700 |
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|a Marmagne, Anne
|e verfasserin
|4 aut
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|a Lebreton, Sandrine
|e verfasserin
|4 aut
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|a Clément, Gilles
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|4 aut
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|a Guivarc'h, Anne
|e verfasserin
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|a Savouré, Arnould
|e verfasserin
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|a Masclaux-Daubresse, Céline
|e verfasserin
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 74(2023), 5 vom: 13. März, Seite 1489-1500
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnas
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| 773 |
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|g volume:74
|g year:2023
|g number:5
|g day:13
|g month:03
|g pages:1489-1500
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|u http://dx.doi.org/10.1093/jxb/erac499
|3 Volltext
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