CAR modulates plasma membrane nano-organization and immune signaling downstream of RALF1-FERONIA signaling pathway

CAR modulates plasma membrane nano-organization and immune signaling downstream of RALF1-FERONIA signaling pathway

© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 237(2023), 6 vom: 14. März, Seite 2148-2162
1. Verfasser: Chen, Weijun (VerfasserIn)
Weitere Verfasser: Zhou, Huina, Xu, Fan, Yu, Meng, Coego, Alberto, Rodriguez, Lesia, Lu, Yuqing, Xie, Qijun, Fu, Qiong, Chen, Jia, Xu, Guoyun, Wu, Dousheng, Li, Xiushan, Li, Xiaojuan, Jaillais, Yvon, Rodriguez, Pedro L, Zhu, Sirui, Yu, Feng
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't C2 domain ABA-related proteins FERONIA immune complex lipid-binding nanodomains Arabidopsis Proteins Phosphotransferases EC 2.7.- mehr... RALF1 protein, Arabidopsis FERONIA receptor like kinase, Arabidopsis
Beschreibung
Zusammenfassung:© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.
In Arabidopsis, the receptor-like kinase (RLK) FERONIA (FER) senses peptide ligands in the plasma membrane (PM), modulates plant growth and development, and integrates biotic and abiotic stress signaling for downstream adaptive responses. However, the molecular interplay of these diverse processes is largely unknown. Here, we show that FER, the receptor of Rapid Alkalinization Factor 1 (RALF1), physically interacts with C2 domain ABA-related (CAR) proteins to control the nano-organization of the PM. During this process, the RALF1-FER pathway upregulates CAR protein translation, and then more CAR proteins are recruited to the PM. This acts as a rapid feedforward loop that stabilizes the PM liquid-ordered phase. FER interacts with and phosphorylates CARs, thereby reducing their lipid-binding ability and breaking the feedback regulation at later time points. The formation of the flg22-induced FLS2-BAK1 immune complex, which depends on the integrity of FER-containing nanodomains, is impaired in fer and pentuple car14569 mutant. Together, we propose that the FER-CAR module controls the formation of PM nano-organization during RALF signaling through a self-contained amplifying loop including both positive and negative feedback
Beschreibung:Date Completed 13.03.2023
Date Revised 13.03.2023
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.18687