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231226s2023 xx |||||o 00| ||eng c |
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|a 10.1002/adma.202208556
|2 doi
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|a pubmed24n1166.xml
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|a (NLM)36493355
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|a DE-627
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|e rakwb
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|a eng
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| 100 |
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|a d'Amone, Luciana
|e verfasserin
|4 aut
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| 245 |
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|a Reshaping de Novo Protein Switches into Bioresponsive Materials for Biomarker, Toxin, and Viral Detection
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|c 2023
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 21.03.2023
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|a Date Revised 21.03.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2023 Wiley-VCH GmbH.
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|a De novo designed protein switches are powerful tools to specifically and sensitively detect diverse targets with simple chemiluminescent readouts. Finding an appropriate material host for de novo designed protein switches without altering their thermodynamics while preserving their intrinsic stability over time would enable the development of a variety of sensing formats to monitor exposure to pathogens, toxins, and for disease diagnosis. Here, a de novo protein-biopolymer hybrid that maintains the detection capabilities induced by the conformational change of the incorporated proteins in response to analytes of interest is generated in multiple, shelf-stable material formats without the need of refrigerated storage conditions. A set of functional demonstrator devices including personal protective equipment such as masks and laboratory gloves, free-standing films, air quality monitors, and wearable devices is presented to illustrate the versatility of the approach. Such formats are designed to be responsive to human epidermal growth factor receptor (HER2), anti-hepatitis B (HBV) antibodies, Botulinum neurotoxin B (BoNT/B), and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This combination of form and function offers wide opportunities for ubiquitous sensing in multiple environments by enabling a large class of bio-responsive interfaces of broad utility
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|a Journal Article
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|a biosensing
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|a de novo design
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| 650 |
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4 |
|a sensing interfaces
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4 |
|a silk fibroin
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| 650 |
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7 |
|a Biomarkers
|2 NLM
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| 700 |
1 |
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|a Matzeu, Giusy
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Quijano-Rubio, Alfredo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Callahan, Gregory P
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Napier, Bradley
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Baker, David
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Omenetto, Fiorenzo G
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Advanced materials (Deerfield Beach, Fla.)
|d 1998
|g 35(2023), 11 vom: 05. März, Seite e2208556
|w (DE-627)NLM098206397
|x 1521-4095
|7 nnns
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| 773 |
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|g volume:35
|g year:2023
|g number:11
|g day:05
|g month:03
|g pages:e2208556
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| 856 |
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|u http://dx.doi.org/10.1002/adma.202208556
|3 Volltext
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