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231226s2023 xx |||||o 00| ||eng c |
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|a 10.1111/jfd.13731
|2 doi
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|a pubmed25n1164.xml
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|a (DE-627)NLM34936334X
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|a (NLM)36423261
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Zhu, Min
|e verfasserin
|4 aut
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| 245 |
1 |
2 |
|a A recombinant baculovirus vector vaccine (BacMCP) against the infectious spleen and kidney necrosis virus (ISKNV)
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| 264 |
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1 |
|c 2023
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 12.01.2023
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|a Date Revised 12.01.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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| 520 |
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|a © 2022 John Wiley & Sons Ltd.
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|a The infectious spleen and kidney necrosis virus (ISKNV) is a highly lethal virus, which has brought significant losses to aquaculture. Therefore, a new vaccine against ISKNV with high efficiency, safety and convenience must be developed. While baculoviruses are more commonly used as protein expression systems for vaccine antigen production, this paper used baculovirus technology to develop a live-vector vaccine, BacMCP, which contains the coding sequence of the major capsid protein (MCP) (GenBank accession no. AF371960) of ISKNV and is driven by a CMV promoter. Real-time PCR and immunofluorescence showed that the MCP gene was successfully delivered to and expressed in fish cells and tissues inoculated with BacMCP. Immune-related gene (IgM, TGF-β, IL-1, IL-8, TNF-α) expression was induced in BacMCP-treated groups of largemouth bass compared with control groups. Specific antibodies could be detected in the serum of BacMCP injection-vaccinated largemouth bass by ELISA. After injection or immersion vaccination with BacMCP for 21 days, largemouth bass were infected with ISKNV. The immune effect of the injected immunization on fish in different sizes was evaluated. The vaccine efficacy of injection-vaccinated bass was 100% in small bass and 85.7% in large bass. The vaccine efficacy of immersion-vaccinated small bass was 77.3%. This study suggested that BacMCP can be used as a vector-based vaccine candidate to prevent the diseases caused by ISKNV infection
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| 650 |
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4 |
|a Journal Article
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|a baculovirus vector vaccine
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| 650 |
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4 |
|a infectious spleen and kidney necrosis virus (ISKNV)
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| 650 |
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4 |
|a largemouth bass
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| 650 |
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7 |
|a Viral Vaccines
|2 NLM
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| 650 |
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7 |
|a Vaccines, Synthetic
|2 NLM
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| 650 |
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7 |
|a Capsid Proteins
|2 NLM
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| 700 |
1 |
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|a Shen, Zeen
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gu, Yuchao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tong, Xinyu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Yaxin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Pan, Jun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Feng, Yongjie
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hu, Xiaolong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Yujun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cao, Guangli
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xue, Renyu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gong, Chengliang
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of fish diseases
|d 1998
|g 46(2023), 2 vom: 24. Feb., Seite 165-176
|w (DE-627)NLM098166034
|x 1365-2761
|7 nnas
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| 773 |
1 |
8 |
|g volume:46
|g year:2023
|g number:2
|g day:24
|g month:02
|g pages:165-176
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| 856 |
4 |
0 |
|u http://dx.doi.org/10.1111/jfd.13731
|3 Volltext
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| 912 |
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|a AR
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| 952 |
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|d 46
|j 2023
|e 2
|b 24
|c 02
|h 165-176
|