Transferring of clubroot-resistant locus CRd from Chinese cabbage (Brassica rapa) to canola (Brassica napus) through interspecific hybridization

Transferring of clubroot-resistant locus CRd from Chinese cabbage (Brassica rapa) to canola (Brassica napus) through interspecific hybridization

Copyright © 2022 by JAPANESE SOCIETY OF BREEDING.

Bibliographische Detailangaben
Veröffentlicht in:Breeding science. - 1998. - 72(2022), 3 vom: 10. Juni, Seite 189-197
1. Verfasser: Zhan, Zongxiang (VerfasserIn)
Weitere Verfasser: Shah, Nadil, Jia, Ru, Li, Xiaonan, Zhang, Chunyu, Piao, Zhongyun
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2022
Zugriff auf das übergeordnete Werk:Breeding science
Schlagworte:Journal Article Brassica napus clubroot resistance interspecific hybridization marker-assisted selection
Beschreibung
Zusammenfassung:Copyright © 2022 by JAPANESE SOCIETY OF BREEDING.
Clubroot, caused by Plasmodiophora brassicae is one of the most severe threats to brassica species in China and worldwide. Breeding for clubroot resistant varieties is one of the best ways to overcome this disease. In this study, we introduced clubroot resistance (CR) gene CRd from Chinese cabbage (85-74) into elite Brassica napus inbred line Zhongshuang 11 through interspecific hybridization and subsequent backcrossing with whole-genome molecular marker-assisted selection (MAS). The resistant test of CRd to P. brassicae isolates was evaluated in the greenhouse as well as in field conditions. Close linkage markers and the whole-chromosome background marker selection approach improved the recovery rate from 78.3% in BC1 to 100% in BC3F1. The improved clubroot-resistant variety, Zhongshuang11R, was successfully selected in the BC3F2 generation. The greenhouse and field resistant tests revealed that Zhongshuang11R was resistant to P. brassicae pathotypes. The agronomic characteristics of Zhongshuang11R were similar to those of its recurrent parental line, including oil content, composition of fatty acid, plant height, primary effective branches, grain yield per plant and thousand-seed weight. In addition, the oil quality could satisfy the quality requirements for commercial rapeseed oil. Our results will enrich the resistant resources of canola and will certainly accelerate clubroot resistance breeding programs in B. napus
Beschreibung:Date Revised 22.11.2022
published: Print-Electronic
Citation Status PubMed-not-MEDLINE
ISSN:1344-7610
DOI:10.1270/jsbbs.21052