Removal of nonspecific binding proteins is required in co-immunoprecipitation with nuclear proteins
Whether protein samples should be pretreated to remove nonspecific binding proteins in co-immunoprecipitation (CO-IP) is controversial. In this work, nonspecific binding of proteins to agarose beads was found to be greater than that to magnetic beads. The nonspecific binding was increased with the d...
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Détails bibliographiques
| Publié dans: | BioTechniques. - 1993. - 73(2022), 6 vom: 01. Dez., Seite 289-296
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| Auteur principal: |
Zeng, Xi
(Auteur) |
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| Autres auteurs: |
Zeng, Wei-Hong,
Zhou, Jun,
Liu, Xiao-Min,
Huang, Guo,
Zhu, Hong,
Xiao, Shuai,
Zeng, Ying,
Cao, Deliang |
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| Format: | Article en ligne
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| Langue: | English |
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| Publié: |
2022
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| Accès à la collection: | BioTechniques
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| Sujets: | Journal Article
Research Support, Non-U.S. Gov't
NP-40
Western blot
agarose beads
co-immunoprecipitation
cytoplasmic and nuclear protein extraction
nonspecific binding proteins
nuclear proteins
osmotic pressure
plus...
preclear
protein–protein interactions
Actins
Carrier Proteins
Nuclear Proteins
Tumor Suppressor Protein p53 |
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