Removal of nonspecific binding proteins is required in co-immunoprecipitation with nuclear proteins
Whether protein samples should be pretreated to remove nonspecific binding proteins in co-immunoprecipitation (CO-IP) is controversial. In this work, nonspecific binding of proteins to agarose beads was found to be greater than that to magnetic beads. The nonspecific binding was increased with the d...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1993. - 73(2022), 6 vom: 01. Dez., Seite 289-296
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| 1. Verfasser: |
Zeng, Xi
(VerfasserIn) |
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| Weitere Verfasser: |
Zeng, Wei-Hong,
Zhou, Jun,
Liu, Xiao-Min,
Huang, Guo,
Zhu, Hong,
Xiao, Shuai,
Zeng, Ying,
Cao, Deliang |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2022
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
NP-40
Western blot
agarose beads
co-immunoprecipitation
cytoplasmic and nuclear protein extraction
nonspecific binding proteins
nuclear proteins
osmotic pressure
mehr...
preclear
protein–protein interactions
Actins
Carrier Proteins
Nuclear Proteins
Tumor Suppressor Protein p53 |
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