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231226s2022 xx |||||o 00| ||eng c |
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7 |
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|a 10.2144/btn-2022-0054
|2 doi
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|a pubmed24n1163.xml
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|a eng
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| 100 |
1 |
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|a Scott, Nathan
|e verfasserin
|4 aut
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| 245 |
1 |
2 |
|a A simplified procedure for isolation of primary murine microglia
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1 |
|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 19.12.2022
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|a Date Revised 22.12.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a There are various approaches in which one can isolate microglia from murine brains, such as immunomagnetic, density gradient, FACS and differential adhesive methods. In this procedure a modified flask-tapping approach was used due to its simplicity and reproducibility. Our protocol requires only a single step to isolate the microglia from the mixed cell population. Once the microglia were isolated, we characterized cell purity, microglial morphology and phagocytic activity. The single-step protocol, without the need for additional astrocyte or oligodendrocyte separation, allows microglial cells to be used immediately for experimental purposes. The protocol is low-cost and can be performed in any lab with standard cell-culture equipment
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4 |
|a Journal Article
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|a Research Support, N.I.H., Extramural
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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4 |
|a flow cytometry
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| 650 |
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4 |
|a fluorescence
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| 650 |
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4 |
|a microglia
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| 650 |
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4 |
|a primary cell
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| 650 |
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4 |
|a tissue culture
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| 700 |
1 |
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|a Witt, Ken
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Schober, Joseph M
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1988
|g 73(2022), 6 vom: 23. Dez., Seite 273-279
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnns
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| 773 |
1 |
8 |
|g volume:73
|g year:2022
|g number:6
|g day:23
|g month:12
|g pages:273-279
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| 856 |
4 |
0 |
|u http://dx.doi.org/10.2144/btn-2022-0054
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