A simple and economical site-directed mutagenesis method for large plasmids by direct transformation of two overlapping PCR fragments

Despite the development of various methods and commercial kits, site-directed mutagenesis of large plasmids remains a challenge in many laboratories. A site-directed mutagenesis method was developed for large plasmids by directly transforming two overlapping PCR fragments into Escherichia coli. This...

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Veröffentlicht in:	BioTechniques. - 1993. - 73(2022), 5 vom: 15. Nov., Seite 239-245
1. Verfasser:	Yang, Zhibo (VerfasserIn)
Weitere Verfasser:	Chen, Zan, Zhang, Yueping
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2022
Zugriff auf das übergeordnete Werk:	BioTechniques
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Escherichia coli Gibson assembly PCR-based site-directed mutagenesis Q5 DNA polymerase Q5 site-directed mutagenesis QuikChange site-directed mutagenesis in vivo assembly in vivo recombination mehr... overlapping extension PCR phusion site-directed mutagenesis DNA-Directed DNA Polymerase EC 2.7.7.7