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NLM349116989 |
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DE-627 |
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20231226041846.0 |
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231226s2023 xx |||||o 00| ||eng c |
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7 |
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|a 10.1002/adma.202207138
|2 doi
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|a pubmed24n1163.xml
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|a (DE-627)NLM349116989
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|a (NLM)36398425
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Song, Minsun
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Multiplexed Ultrasensitive Sample-to-Answer RT-LAMP Chip for the Identification of SARS-CoV-2 and Influenza Viruses
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|c 2023
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|a Text
|b txt
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 14.03.2023
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|a Date Revised 14.03.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2023 The Authors. Advanced Materials published by Wiley-VCH GmbH.
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|a Prompt on-site diagnosis of SARS-CoV-2 with other respiratory infections will have minimized the global impact of the COVID-19 pandemic through rapid, effective management. However, no such multiplex point-of-care (POC) chip has satisfied a suitable sensitivity of gold-standard nucleic acid amplification tests (NAATs). Here, a rapid multiplexed ultrasensitive sample-to-answer loop-mediated isothermal amplification (MUSAL) chip operated by simple LED-driven photothermal amplification to detect six targets from single-swab sampling is presented. First, the MUSAL chip allows ultrafast on-chip sample preparation with ≈500-fold preconcentration at a rate of 1.2 mL min-1 . Second, the chip enables contamination-free amplification using autonomous target elution into on-chip reagents by photothermal activation. Finally, the chip accomplishes multiplexed on-chip diagnostics of SARS-CoV-2 and influenza viruses with a limit of detection (LoD) of 0.5 copies µL-1 . The rapid, ultrasensitive, cost-effective sample-to-answer chip with a multiplex capability will allow timely management of various pandemics situations that may be faced shortly
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|a Journal Article
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|a SARS-CoV-2
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|a multiplexing
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|a on-chip sample preparation
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4 |
|a preconcentration
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| 650 |
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4 |
|a reverse transcription loop-mediated isothermal amplification
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| 650 |
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4 |
|a sample-to-answer
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| 650 |
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4 |
|a ultrasensitive point-of-care chips
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| 700 |
1 |
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|a Hong, SoonGweon
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lee, Luke P
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Advanced materials (Deerfield Beach, Fla.)
|d 1998
|g 35(2023), 10 vom: 17. März, Seite e2207138
|w (DE-627)NLM098206397
|x 1521-4095
|7 nnns
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| 773 |
1 |
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|g volume:35
|g year:2023
|g number:10
|g day:17
|g month:03
|g pages:e2207138
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| 856 |
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|u http://dx.doi.org/10.1002/adma.202207138
|3 Volltext
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