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NLM348973675 |
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DE-627 |
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20231226041543.0 |
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cr uuu---uuuuu |
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231226s2023 xx |||||o 00| ||eng c |
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7 |
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|a 10.1094/PDIS-08-22-1970-RE
|2 doi
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|a pubmed24n1163.xml
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|a (DE-627)NLM348973675
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|a (NLM)36383991
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Jin, Weijie
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Development of Colloidal Gold Immunochromatography and Reverse-Transcription Loop-Mediated Isothermal Amplification Assays to Detect Lychnis Mottle Virus
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|c 2023
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 02.06.2023
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|a Date Revised 02.06.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Lychnis mottle virus (LycMoV; genus Unassigned, family Secoviridae) infection of Angelica sinensis produces mottle and mosaic symptoms, damaging the host. Early detection of relevant pathogens is the most critical step in preventing the potential transmission of infectious disease. Polyclonal antibodies with high potency and high specificity were prepared using the recombinant LycMoV capsid protein as an antigen. Here, we developed and optimized a rapid colloidal gold immunochromatography assay (GICA) detection system for LycMoV using this antibody. Under optimum conditions, GICA specifically detected (up to 10,000-fold) positive LycMoV samples. A real-time reverse-transcription loop-mediated isothermal amplification (RT-LAMP) system was also established by selecting the primers with high sensitivity and specificity to LycMoV. The RT-LAMP detection threshold was 1.42 fg/μl (291 copies/μl). A GICA-RT-LAMP assay system was further established and optimized. The minimum GICA detection line was calculated at 1.52 × 10-2 ng/μl. Although GICA did not detect positive samples after capturing virus at 2.53 × 10-3 ng/μl, GICA-LAMP and GICA-RT-PCR did, whose sensitivity was comparatively greater than sixfold. This is the first report showing that GICA-RT-LAMP is a cost-effective approach for use in detecting LycMoV without extracting nucleic acids. These sensitive assays will help improve virus disease management in A. sinensis crops
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a Angelica sinensis (Oliv.) Diels
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| 650 |
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4 |
|a Lychnis mottle virus
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| 650 |
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4 |
|a RT-LAMP
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| 650 |
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4 |
|a RT-PCR
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| 650 |
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4 |
|a immunochromatographic assay
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| 650 |
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7 |
|a Gold Colloid
|2 NLM
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| 700 |
1 |
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|a Zhang, Yubao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Su, Xuesi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Ruoyu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xie, Zhongkui
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Yajun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Qiu, Yang
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Plant disease
|d 1997
|g 107(2023), 5 vom: 16. Mai, Seite 1584-1592
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnns
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| 773 |
1 |
8 |
|g volume:107
|g year:2023
|g number:5
|g day:16
|g month:05
|g pages:1584-1592
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| 856 |
4 |
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|u http://dx.doi.org/10.1094/PDIS-08-22-1970-RE
|3 Volltext
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| 912 |
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|a GBV_USEFLAG_A
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| 912 |
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|a SYSFLAG_A
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|a GBV_NLM
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| 951 |
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|a AR
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| 952 |
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|d 107
|j 2023
|e 5
|b 16
|c 05
|h 1584-1592
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