Population Genetic Analysis of Fusarium decemcellulare, a Guaraná Pathogen, Reveals High Genetic Diversity in the Amazonas State, Brazil

Guaraná is indigenous to the Brazilian Amazon where it has cultural and agroeconomic significance. However, its cultivation is constrained by a disease termed oversprouting of guaraná caused by Fusarium decemcellulare, with yield losses reaching as high as 100%. The disease can affect different part...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:Plant disease. - 1997. - 107(2023), 5 vom: 09. Mai, Seite 1343-1354
1. Verfasser: Queiroz, Claudia A (VerfasserIn)
Weitere Verfasser: Caniato, Fernanda F, Siqueira, Vanessa K S, de Moraes Catarino, Aricléia, Hanada, Rogério E, O'Donnell, Kerry, Laraba, Imane, Rehner, Stephen A, Sousa, Nelcimar Reis, Silva, Gilvan F
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:Plant disease
Schlagworte:Journal Article ISSR RPB1 RPB2 TEF1 hyperplasia hypertrophy mating type oversprouting phylogenetics stem galls
LEADER 01000naa a22002652 4500
001 NLM348644264
003 DE-627
005 20231226040740.0
007 cr uuu---uuuuu
008 231226s2023 xx |||||o 00| ||eng c
024 7 |a 10.1094/PDIS-01-22-0083-RE  |2 doi 
028 5 2 |a pubmed24n1162.xml 
035 |a (DE-627)NLM348644264 
035 |a (NLM)36350732 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Queiroz, Claudia A  |e verfasserin  |4 aut 
245 1 0 |a Population Genetic Analysis of Fusarium decemcellulare, a Guaraná Pathogen, Reveals High Genetic Diversity in the Amazonas State, Brazil 
264 1 |c 2023 
336 |a Text  |b txt  |2 rdacontent 
337 |a ƒaComputermedien  |b c  |2 rdamedia 
338 |a ƒa Online-Ressource  |b cr  |2 rdacarrier 
500 |a Date Completed 02.06.2023 
500 |a Date Revised 02.06.2023 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a Guaraná is indigenous to the Brazilian Amazon where it has cultural and agroeconomic significance. However, its cultivation is constrained by a disease termed oversprouting of guaraná caused by Fusarium decemcellulare, with yield losses reaching as high as 100%. The disease can affect different parts of the plant, causing floral hypertrophy and hyperplasia, stem galls, and oversprouting of vegetative buds. To date, no study has been conducted characterizing the genetic diversity and population structure of this pathogen. Here, we report genetic diversity and genetic structure among 224 isolates from eight guaraná production areas of Amazonas State, Brazil, that were genotyped using a set of 10 inter-simple-sequence repeat (ISSR) markers. Despite moderate gene diversity (Hexp = 0.21 to 0.32), genotypic diversity was at or near maximum (223 multilocus genotypes among 224 isolates). Population genetic analysis of the 10 ISSR marker fragments with STRUCTURE software identified two populations designated C1 and C2 within the F. decemcellulare collection from the eight sites. Likewise, UPGMA hierarchical clustering and discriminant analysis of principal components of the strains from guaraná resolved these same two groups. Analysis of molecular variance demonstrated that 71% of genetic diversity occurred within the C1 and C2 populations. A pairwise comparison of sampling sites for both genetic populations revealed that 59 of 66 were differentiated from one another (P < 0.05), and high and significant gene flow was detected only between sampling sites assigned to the same genetic population. The presence of MAT1-1 and MAT1-2 strains, in conjunction with the high genotypic diversity and no significant linkage disequilibrium, suggests that each population of F. decemcellulare might be undergoing sexual reproduction. Isolation by distance was not observed (R2 = 0.02885, P > 0.05), which suggests that human-mediated movement of seedlings may have played a role in shaping the F. decemcellulare genetic structure in Amazonas State, Brazil 
650 4 |a Journal Article 
650 4 |a ISSR 
650 4 |a RPB1 
650 4 |a RPB2 
650 4 |a TEF1 
650 4 |a hyperplasia 
650 4 |a hypertrophy 
650 4 |a mating type 
650 4 |a oversprouting 
650 4 |a phylogenetics 
650 4 |a stem galls 
700 1 |a Caniato, Fernanda F  |e verfasserin  |4 aut 
700 1 |a Siqueira, Vanessa K S  |e verfasserin  |4 aut 
700 1 |a de Moraes Catarino, Aricléia  |e verfasserin  |4 aut 
700 1 |a Hanada, Rogério E  |e verfasserin  |4 aut 
700 1 |a O'Donnell, Kerry  |e verfasserin  |4 aut 
700 1 |a Laraba, Imane  |e verfasserin  |4 aut 
700 1 |a Rehner, Stephen A  |e verfasserin  |4 aut 
700 1 |a Sousa, Nelcimar Reis  |e verfasserin  |4 aut 
700 1 |a Silva, Gilvan F  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Plant disease  |d 1997  |g 107(2023), 5 vom: 09. Mai, Seite 1343-1354  |w (DE-627)NLM098181742  |x 0191-2917  |7 nnns 
773 1 8 |g volume:107  |g year:2023  |g number:5  |g day:09  |g month:05  |g pages:1343-1354 
856 4 0 |u http://dx.doi.org/10.1094/PDIS-01-22-0083-RE  |3 Volltext 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_350 
951 |a AR 
952 |d 107  |j 2023  |e 5  |b 09  |c 05  |h 1343-1354