Enhanced electrochemical measurement of β-galactosidase activity in whole cells by coexpression of lactose permease, LacY
Whole-cell biosensing links the sensing and computing capabilities of microbes to the generation of a detectable reporter. Whole cells enable dynamic biological computation (filtered noise, amplified signals, logic gating etc.). Enzymatic reporters enable in situ signal amplification. Electrochemica...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1988. - 73(2022), 5 vom: 08. Nov., Seite 233-237
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| 1. Verfasser: |
VanArsdale, Eric
(VerfasserIn) |
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| Weitere Verfasser: |
Pitzer, Juliana,
Wang, Sally,
Stephens, Kristina,
Chen, Chen-Yu,
Payne, Gregory F,
Bentley, William E |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2022
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
Miller assay
electrochemical sensing
synthetic biology
whole-cell β-galactosidase assay
lactose permease
9068-45-5
Membrane Transport Proteins
beta-Galactosidase
mehr...
EC 3.2.1.23
Galactose
X2RN3Q8DNE
Escherichia coli Proteins
LacY protein, E coli
Monosaccharide Transport Proteins
Symporters |
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