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231226s2023 xx |||||o 00| ||eng c |
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|a 10.1016/j.plantsci.2022.111509
|2 doi
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|a pubmed25n1159.xml
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|a (DE-627)NLM347981119
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|a (NLM)36283579
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|a (PII)S0168-9452(22)00334-X
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|a DE-627
|b ger
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Peng, Yuquan
|e verfasserin
|4 aut
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| 245 |
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|a Transcriptomic and functional characterization reveals CsHAK5;3 as a key player in K+ homeostasis in grafted cucumbers under saline conditions
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|c 2023
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 16.12.2022
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|a Date Revised 20.12.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2022 Elsevier B.V. All rights reserved.
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|a Grafting can improve the salt tolerance of many crops. However, critical genes in scions responsive to rootstock under salt stress remain a mystery. We found that pumpkin rootstock decreased the content of Na+ by 70.24 %, increased the content of K+ by 25.9 %, and increased the K+/Na+ ratio by 366.0 % in cucumber scion leaves. RNA-seq analysis showed that ion transport-related genes were the key genes involved in salt stress tolerance in grafted cucumber. The identification and analysis of the expression of K+ transporter proteins in cucumber and pumpkin revealed six and five HAK5 members, respectively. The expression of CsHAK5;3 in cucumber was elevated in different graft combinations under salt stress and most notably in cucumber scion/pumpkin rootstock. CsHAK5;3 was localized to the plasma membrane, and a yeast complementation assay revealed that it can transport K+. CsHAK5;3 knockout in hairy root mutants decreased the K+ content of leaves (45.6 %) and roots (50.3 %), increased the Na+ content of leaves (29.3 %) and roots (34.8 %), and decreased the K+/Na+ ratio of the leaves (57.9 %) and roots (62.9 %) in cucumber. However, CsHAK5;3 overexpression in hairy roots increased the K+ content of the leaves (31.2 %) and roots (38.3 %), decreased the Na+ content of leaves (17.2 %) and roots (14.3 %), and increased the K+/Na+ ratio of leaves (58.9 %) and roots (61.6 %) in cucumber. In conclusion, CsHAK5;3 in cucumber can mediate K+ transport and is one of the key target pumpkin genes that enhance salt tolerance of cucumber grafted
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|a Journal Article
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|a Grafted cucumber
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|a HAK5
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|a K(+) homeostasis
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|a Salinity
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|a Transcriptome
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| 650 |
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|a Sodium
|2 NLM
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| 650 |
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|a 9NEZ333N27
|2 NLM
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| 700 |
1 |
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|a Cao, Haishun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Peng, Zhaowen
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhou, Lijian
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sohail, Hamza
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cui, Lvjun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yang, Li
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Huang, Yuan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bie, Zhilong
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 326(2023) vom: 13. Jan., Seite 111509
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnas
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| 773 |
1 |
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|g volume:326
|g year:2023
|g day:13
|g month:01
|g pages:111509
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|u http://dx.doi.org/10.1016/j.plantsci.2022.111509
|3 Volltext
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