A jasmonate-responsive bHLH transcription factor TaMYC2 positively regulates triterpenes biosynthesis in Taraxacum antungense Kitag

Copyright © 2022 Elsevier B.V. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant science : an international journal of experimental plant biology. - 1985. - 326(2023) vom: 13. Jan., Seite 111506
1. Verfasser: Liu, Tingyao (VerfasserIn)
Weitere Verfasser: Liao, Jingjing, Shi, Min, Li, Li, Liu, Qun, Cui, Xin, Ning, Wei, Kai, Guoyin
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:Plant science : an international journal of experimental plant biology
Schlagworte:Journal Article Biosynthesis MYC2 Taraxacum antungense Kitag Transcriptional regulation Triterpenes Basic Helix-Loop-Helix Transcription Factors jasmonic acid 6RI5N05OWW methyl jasmonate mehr... 900N171A0F Oxylipins Cyclopentanes taraxasterol 64SK2ERN9P taraxerol A32778O852
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520 |a Dandelion is a well-known traditional medical herb, also used as functional food. Dandelion possesses many medical properties, such as anti-bacterial and antioxidant activity and contains a variety of triterpenes, such as α-amyrin, β-amyrin, taraxerol and taraxasterol. In this study, we found that triterpenes biosynthesis was promoted by methyl jasmonate (MeJA), while the transcriptional mechanism underlying triterpenes biosynthesis was rarely investigated. Here, a MeJA-induced bHLH transcription factor TaMYC2 was identified. The content of taraxasterol and taraxerol in dandelion was obviously enhanced in overexpression TaMYC2 transgenic lines and expression level of the squalene synthase gene (TaSS) was elevated to about 3-5 folds compared with the control lines. Dual-LUC, Y1H and EMSA experiments revealed that TaMYC2 bound to the E-box motif in the promoter of TaSS and activated its transcription. Taken together, this study suggested that TaMYC2 acted as a positive regulator for bioengineering approaches to produce high content triterpenes-producing dandelions 
650 4 |a Journal Article 
650 4 |a Biosynthesis 
650 4 |a MYC2 
650 4 |a Taraxacum antungense Kitag 
650 4 |a Transcriptional regulation 
650 4 |a Triterpenes 
650 7 |a Basic Helix-Loop-Helix Transcription Factors  |2 NLM 
650 7 |a jasmonic acid  |2 NLM 
650 7 |a 6RI5N05OWW  |2 NLM 
650 7 |a methyl jasmonate  |2 NLM 
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650 7 |a Cyclopentanes  |2 NLM 
650 7 |a taraxasterol  |2 NLM 
650 7 |a 64SK2ERN9P  |2 NLM 
650 7 |a Triterpenes  |2 NLM 
650 7 |a taraxerol  |2 NLM 
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700 1 |a Liao, Jingjing  |e verfasserin  |4 aut 
700 1 |a Shi, Min  |e verfasserin  |4 aut 
700 1 |a Li, Li  |e verfasserin  |4 aut 
700 1 |a Liu, Qun  |e verfasserin  |4 aut 
700 1 |a Cui, Xin  |e verfasserin  |4 aut 
700 1 |a Ning, Wei  |e verfasserin  |4 aut 
700 1 |a Kai, Guoyin  |e verfasserin  |4 aut 
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773 1 8 |g volume:326  |g year:2023  |g day:13  |g month:01  |g pages:111506 
856 4 0 |u http://dx.doi.org/10.1016/j.plantsci.2022.111506  |3 Volltext 
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