Comprehensive analysis of B and T cell receptor repertoire in patients after kidney transplantation by high-throughput sequencing

Comprehensive analysis of B and T cell receptor repertoire in patients after kidney transplantation by high-throughput sequencing

Copyright © 2022. Published by Elsevier Inc.

Bibliographische Detailangaben
Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 245(2022) vom: 25. Dez., Seite 109162
1. Verfasser: Wang, Guangyu (VerfasserIn)
Weitere Verfasser: Sui, Weiguo, Xue, Wen, Zhang, Junning, Yang, Xueli, Mo, Chune, Pan, Xiaoping, Ou, Minglin, Hou, Xianliang
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2022
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Journal Article Research Support, Non-U.S. Gov't B cells Complementarity determining regions High-throughput sequencing Immune repertoire Kidney transplantation T cells Complementarity Determining Regions Receptors, Antigen, B-Cell Receptors, Antigen, T-Cell
Beschreibung
Zusammenfassung:Copyright © 2022. Published by Elsevier Inc.
PURPOSE: The dynamic immunity of kidney transplant patients has not been fully elucidated. In this study, we explored the repertoire features of B/T cell receptor (BCR/TCR) of kidney transplant patients
METHODS: Using combined multiplex PCR amplification and high-throughput sequencing technique, we analyzed the uremic patients' BCR H chain and TCR beta chain repertoire which obtained 1 day before kidney transplantation (PRE-1), 1 day and 7 day after kidney transplantation (POST-1 and POST-7)
RESULTS: Our analysis results showed the diversity of TCRβ CDR3 in POST-7 group was highest. In addition, there were specific skewed usage of TRBV gene subfamilies, and V-J combinations in different time points during kidney transplantation. Moreover, the overlap degrees of BCR-H (TCR-β) CDR3 repertoire among each group were identified. Notably, the abundance of some TCR-β CDR3 sequences changed regularly in the time point of kidney transplantation
CONCLUSIONS: The BCR-H (TCR-β) CDR3 repertoire of kidney transplant patients changed dynamically
Beschreibung:Date Completed 28.11.2022
Date Revised 26.12.2022
published: Print-Electronic
Citation Status MEDLINE
ISSN:1521-7035
DOI:10.1016/j.clim.2022.109162