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231226s2022 xx |||||o 00| ||eng c |
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|a 10.1016/j.plantsci.2022.111496
|2 doi
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|a pubmed24n1158.xml
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|a (DE-627)NLM347559387
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|a (NLM)36240910
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|a (PII)S0168-9452(22)00321-1
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Wang, Limin
|e verfasserin
|4 aut
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| 245 |
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|a MdCo31 interacts with an RNA polymerase II transcription subunit 32 to regulate dwarf growth with short internodes in columnar apple
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 01.11.2022
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|a Date Revised 01.11.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2022 Elsevier B.V. All rights reserved.
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|a The dominant Co locus controls the columnar growth phenotype of apple (Malus × domestica) trees. Candidate gene MdCo31, encoding 2-oxoglutarate-dependent dioxygenase, causes dwarf growth with short internodes in transgenic plants by reducing the abundance of biologically active gibberellin. However, the pathway regulating MdCo31 in the dwarfism of apple trees remains unclear. In this study, expression of MdCo31 was proved to be negatively correlated with internode length in F1 populations created by crossing columnar parents, and with dwarfism in transgenic apple plantlets. Yeast (Saccharomyces cerevisiae) two-hybrid screening identified the RNA polymerase II transcription subunit MdMED32 as putative interactor of MdCo31. Bimolecular fluorescence complementation, co-immunoprecipitation, and dual-luciferase reporter assays confirmed this interaction both in vivo and in vitro. Ectopic expression of MdMED32 in Nicotiana tabacum led to a dwarf phenotype, similar to that of MdCo31 transgenic apple plants. Expression of GA2ox1 and GA20ox1, encoding key enzymes of gibberellin metabolism, was upregulated in transgenic plants. Transient transcriptional activity demonstrated that MdMED32 functioned as an activator, promoting expression of MdGA2ox1 and MdGA20ox1. These findings indicate that the interaction between MdCo31 and MdMED32 functions in the regulation of internode length in columnar apple
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|a Journal Article
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|a Columnar apple
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|a Dwarf growth
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|a MdCo31
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|a MdMED32
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|a Short internode
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|a Transgenic apple
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|a Gibberellins
|2 NLM
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| 650 |
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|a RNA Polymerase II
|2 NLM
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| 650 |
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|a EC 2.7.7.-
|2 NLM
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| 650 |
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|a Plant Proteins
|2 NLM
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| 700 |
1 |
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|a Guo, Jing
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chu, Yu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Pan, Qi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhu, Yuandi
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 325(2022) vom: 12. Dez., Seite 111496
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnns
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| 773 |
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|g volume:325
|g year:2022
|g day:12
|g month:12
|g pages:111496
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|u http://dx.doi.org/10.1016/j.plantsci.2022.111496
|3 Volltext
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