OsPHS1 is required for both male and female gamete development in rice

OsPHS1 is required for both male and female gamete development in rice

Copyright © 2022 Elsevier B.V. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant science : an international journal of experimental plant biology. - 1985. - 325(2022) vom: 05. Dez., Seite 111480
1. Verfasser: Yu, Hao (VerfasserIn)
Weitere Verfasser: Zhang, Liping, He, Xiaojuan, Zhang, Taohui, Wang, Chaolong, Lu, Jiayu, He, Xiaodong, Chen, Keyi, Gu, Weihang, Cheng, Siqi, Hu, Yang, Yao, Bowen, Jian, Anqi, Yu, Xiaowen, Zheng, Hai, You, Shimin, Wang, Qiming, Lei, Dekun, Jiang, Ling, Zhao, Zhigang, Wan, Jianmin
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2022
Zugriff auf das übergeordnete Werk:Plant science : an international journal of experimental plant biology
Schlagworte:Journal Article Chromosome synapsis Homologous chromosome pairing Meiosis Sterility Plant Proteins
LEADER 01000naa a22002652 4500
001 NLM346993105
003 DE-627
005 20231226032754.0
007 cr uuu---uuuuu
008 231226s2022 xx |||||o 00| ||eng c
024 7 |a 10.1016/j.plantsci.2022.111480  |2 doi 
028 5 2 |a pubmed24n1156.xml 
035 |a (DE-627)NLM346993105 
035 |a (NLM)36183810 
035 |a (PII)S0168-9452(22)00305-3 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Yu, Hao  |e verfasserin  |4 aut 
245 1 0 |a OsPHS1 is required for both male and female gamete development in rice 
264 1 |c 2022 
336 |a Text  |b txt  |2 rdacontent 
337 |a ƒaComputermedien  |b c  |2 rdamedia 
338 |a ƒa Online-Ressource  |b cr  |2 rdacarrier 
500 |a Date Completed 01.11.2022 
500 |a Date Revised 01.11.2022 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a Copyright © 2022 Elsevier B.V. All rights reserved. 
520 |a Meiosis plays an essential role in the production of male and female gametes. Extensive studies have elucidated that homologous chromosome association and pairing are essential for crossing-over and recombination of chromosomal segments. However, the molecular mechanism of chromosome recognition and pairing remains elusive. Here, we identified a rice male-female sterility mutant plant. Cytological observations showed that the development of both pollen and embryo sacs of the mutant were abnormal due to defects in homologous chromosome recognition and pairing during prophase I. Map-based cloning revealed that Os06g0473000 encoding a poor homologous synapsis 1 (PHS1) protein is the candidate target gene, which was confirmed by knockout using CRISPR/Cas9 technology. Sequence analysis revealed a single base mutation (G > A) involving the junction of the fourth exon and intron of OsPHS1, which is predicted to alter splicing, resulting in an Osphs1 mutant. Expression pattern analysis indicated that OsPHS1 expression levels were mainly expressed in panicles at the beginning of meiosis. Subcellular localization analysis demonstrated that the OsPHS1 protein is situated in the nucleus and cytoplasm. Taken together, our results suggest an important role for OsPHS1 in homologous chromosome pairing in both male and female gametogenesis in rice 
650 4 |a Journal Article 
650 4 |a Chromosome synapsis 
650 4 |a Homologous chromosome pairing 
650 4 |a Meiosis 
650 4 |a Sterility 
650 7 |a Plant Proteins  |2 NLM 
700 1 |a Zhang, Liping  |e verfasserin  |4 aut 
700 1 |a He, Xiaojuan  |e verfasserin  |4 aut 
700 1 |a Zhang, Taohui  |e verfasserin  |4 aut 
700 1 |a Wang, Chaolong  |e verfasserin  |4 aut 
700 1 |a Lu, Jiayu  |e verfasserin  |4 aut 
700 1 |a He, Xiaodong  |e verfasserin  |4 aut 
700 1 |a Chen, Keyi  |e verfasserin  |4 aut 
700 1 |a Gu, Weihang  |e verfasserin  |4 aut 
700 1 |a Cheng, Siqi  |e verfasserin  |4 aut 
700 1 |a Hu, Yang  |e verfasserin  |4 aut 
700 1 |a Yao, Bowen  |e verfasserin  |4 aut 
700 1 |a Jian, Anqi  |e verfasserin  |4 aut 
700 1 |a Yu, Xiaowen  |e verfasserin  |4 aut 
700 1 |a Zheng, Hai  |e verfasserin  |4 aut 
700 1 |a You, Shimin  |e verfasserin  |4 aut 
700 1 |a Wang, Qiming  |e verfasserin  |4 aut 
700 1 |a Lei, Dekun  |e verfasserin  |4 aut 
700 1 |a Jiang, Ling  |e verfasserin  |4 aut 
700 1 |a Zhao, Zhigang  |e verfasserin  |4 aut 
700 1 |a Wan, Jianmin  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Plant science : an international journal of experimental plant biology  |d 1985  |g 325(2022) vom: 05. Dez., Seite 111480  |w (DE-627)NLM098174193  |x 1873-2259  |7 nnns 
773 1 8 |g volume:325  |g year:2022  |g day:05  |g month:12  |g pages:111480 
856 4 0 |u http://dx.doi.org/10.1016/j.plantsci.2022.111480  |3 Volltext 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_350 
951 |a AR 
952 |d 325  |j 2022  |b 05  |c 12  |h 111480