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231226s2022 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.2c02261
|2 doi
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|a (NLM)36154016
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|a DE-627
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Fabiunke, Simon
|e verfasserin
|4 aut
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| 245 |
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|a Unitary Response of Solvatochromic Dye to Pulse Excitation in Lipid and Cell Membranes
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 12.10.2022
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|a Date Revised 13.10.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The existence of acoustic pulse propagation in lipid monolayers at the air-water interface is well known. These pulses are controlled by the thermodynamic state of the lipid membrane. Nevertheless, the role of acoustic pulses for intra- and inter-cellular communication is still a matter of debate. Herein, we used the dye di-4-ANEPPDHQ, which is known to be sensitive to the physical state and transmembrane potential of membranes, in order to gain insights into compression waves in lipid-based membrane interfaces. The dye was incorporated into lipid monolayers made of phosphatidylserine or phosphatidylcholine at the air-water-interface. A significant blue shift of the emission spectrum was detected when the state of the monolayer was changed from the liquid-expanded (LE) to the liquid-condensed (LC) phase. This "transition sensitivity" of di-4-ANEPPDHQ was generalized in experiments with the bulk solvent dimethyl sulfoxide (DMSO). Upon crystallization of solvent, the emission spectrum also underwent a blue shift. During compression pulses in lipid monolayers, a significant fluorescence response was only observed when the main transition is crossed. The optical signature of these waves─in terms of sign and magnitude─was identical to the response of di-4-ANEPPDHQ during action potentials in neurons and excitable plant cells. These findings corroborated the suggestion that action potentials are nonlinear state changes that propagate in the cell membrane
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Phosphatidylcholines
|2 NLM
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|a Phosphatidylserines
|2 NLM
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|a Solvents
|2 NLM
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|a Water
|2 NLM
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|a 059QF0KO0R
|2 NLM
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|a Dimethyl Sulfoxide
|2 NLM
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|a YOW8V9698H
|2 NLM
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1 |
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|a Fillafer, Christian
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Schneider, Matthias F
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1985
|g 38(2022), 40 vom: 11. Okt., Seite 12408-12413
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnas
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| 773 |
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|g volume:38
|g year:2022
|g number:40
|g day:11
|g month:10
|g pages:12408-12413
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|u http://dx.doi.org/10.1021/acs.langmuir.2c02261
|3 Volltext
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