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231226s2023 xx |||||o 00| ||eng c |
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|a 10.1111/jfd.13716
|2 doi
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|a pubmed24n1154.xml
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|a (DE-627)NLM346465028
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|a (NLM)36130072
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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1 |
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|a Inohana, Mari
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Genital mycobacteriosis caused by Mycobacterium marinum detected in two captive sharks by peptide nucleic acid-fluorescence in situ hybridization
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|c 2023
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 15.12.2022
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|a Date Revised 13.04.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2022 The Authors. Journal of Fish Diseases published by John Wiley & Sons Ltd.
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|a Mycobacterium marinum is a prevalent nontuberculous mycobacterium (NTM)-infecting teleosts. Conversely, little is known about mycobacteriosis in elasmobranchs, and M. marinum infection has never been reported from the subclass. This study investigated the histopathological characteristics and localization of this mycobacterium through molecular analysis of two captive sharks, a scalloped hammerhead Sphyrna lewini and a Japanese bullhead shark Heterodontus japonicus, exhibited in the same aquarium tank. We detected genital mycobacteriosis caused by M. marinum infection using molecular analyses, including polymerase chain reaction (PCR) and DNA sequencing targeting the 60 kDa heat-shock protein gene (hsp65), and peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) targeting the 16S rRNA gene. Both sharks showed granulomas in connective tissues of the gonads without central necrosis or surrounding fibrous capsules, which is unlike the typical mycobacterial granulomas seen in teleosts. This study reveals that elasmobranchs can be aquatic hosts of M. marinum. Because M. marinum is a representative waterborne NTM and a potential zoonotic agent, cautious and intensive research is needed to overcome a lack of data on the relationship between NTM and the aquatic environment in association with this subclass of Chondrichthyes
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|a Journal Article
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|a Mycobacterium marinum
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|a PNA-FISH
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|a elasmobranch
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|a mycobacteriosis
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|a zoonosis
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| 650 |
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|a Peptide Nucleic Acids
|2 NLM
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| 650 |
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|a RNA, Ribosomal, 16S
|2 NLM
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| 700 |
1 |
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|a Komine, Takeshi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tanaka, Yoshiaki
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kurata, Osamu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wada, Shinpei
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Journal of fish diseases
|d 1998
|g 46(2023), 1 vom: 24. Jan., Seite 47-59
|w (DE-627)NLM098166034
|x 1365-2761
|7 nnns
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| 773 |
1 |
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|g volume:46
|g year:2023
|g number:1
|g day:24
|g month:01
|g pages:47-59
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|u http://dx.doi.org/10.1111/jfd.13716
|3 Volltext
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