Pseudouridylation of chloroplast ribosomal RNA contributes to low temperature acclimation in rice

Bibliographische Detailangaben
Veröffentlicht in:	The New phytologist. - 1979. - 236(2022), 5 vom: 29. Dez., Seite 1708-1720
1. Verfasser:	Wang, Zhen (VerfasserIn)
Weitere Verfasser:	Sun, Jing, Zu, Xiaofeng, Gong, Jie, Deng, Hongjing, Hang, Runlai, Zhang, Xiaofan, Liu, Chunyan, Deng, Xian, Luo, Lilan, Wei, Xiangjin, Song, Xianwei, Cao, Xiaofeng
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2022
Zugriff auf das übergeordnete Werk:	The New phytologist
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't chloroplast pseudouridine synthase reactive oxygen species (ROS) ribosome rice temperature RNA, Chloroplast RNA, Ribosomal

Beschreibung
Zusammenfassung:	© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation. Ribosomal RNAs (rRNAs) undergo many modifications during transcription and maturation; homeostasis of rRNA modifications is essential for chloroplast biogenesis in plants. The chloroplast acts as a hub to sense environmental signals, such as cold temperature. However, how RNA modifications contribute to low temperature responses remains unknown. Here we reveal that pseudouridine (Ψ) modification of rice chloroplast rRNAs mediated by the pseudouridine synthase (OsPUS1) contributes to cold tolerance at seedling stage. Loss-function of OsPUS1 leads to abnormal chloroplast development and albino seedling phenotype at low temperature. We find that OsPUS1 is accumulated upon cold and binds to chloroplast precursor rRNAs (pre-rRNAs) to catalyse the pseudouridylation on rRNA. These modifications on chloroplast rRNAs could be required for their processing, as the reduction of mature chloroplast rRNAs and accumulation of pre-rRNAs are observed in ospus1-1 at low temperature. Therefore, the ribosome activity and translation in chloroplasts is disturbed in ospus1-1. Furthermore, transcriptome and translatome analysis reveals that OsPUS1 balances growth and stress-responsive state, preventing excess reactive oxygen species accumulation. Taken together, our findings unveil a crucial function of Ψ in chloroplast ribosome biogenesis and cold tolerance in rice, with potential applications in crop improvement
Beschreibung:	Date Completed 18.11.2022 Date Revised 14.12.2022 published: Print-Electronic Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.18479