|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM345841638 |
| 003 |
DE-627 |
| 005 |
20231226030008.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231226s2022 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1093/jxb/erac359
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n1152.xml
|
| 035 |
|
|
|a (DE-627)NLM345841638
|
| 035 |
|
|
|a (NLM)36067007
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Demidov, Dmitri
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Haploid induction by nanobody-targeted ubiquitin-proteasome-based degradation of EYFP-tagged CENH3 in Arabidopsis thaliana
|
| 264 |
|
1 |
|c 2022
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 15.12.2022
|
| 500 |
|
|
|a Date Revised 21.12.2022
|
| 500 |
|
|
|a published: Print
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a © The Author(s) 2022. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.
|
| 520 |
|
|
|a The generation of haploid plants accelerates the crop breeding process. One of the haploidization strategies is based on the genetic manipulation of endogenous centromere-specific histone 3 (CENH3). To extend the haploidization toolbox, we tested whether targeted in vivo degradation of CENH3 protein can be harnessed to generate haploids in Arabidopsis thaliana. We show that a recombinant anti-GFP nanobody fused to either heterologous F-box (NSlmb) or SPOP/BTB ligase proteins can recognize maternally derived enhanced yellow fluorescent protein (EYFP)-tagged CENH3 in planta and make it accessible for the ubiquitin-proteasome pathway. Outcrossing of the genomic CENH3-EYFP-complemented cenh3.1 mother with plants expressing the GFP-nanobody-targeted E3 ubiquitin ligase resulted in a haploid frequency of up to 7.6% in pooled F1 seeds. EYFP-CENH3 degradation occurred independently in embryo and endosperm cells. In reciprocal crosses, no haploid induction occurred. We propose that the uniparental degradation of EYFP-fused genomic CENH3 during early embryogenesis leads to a decrease in its level at centromeres and subsequently weakens the centromeres. The male-derived wild type CENH3 containing centromere outcompetes the CENH3-EYFP depleted centromere. Consequently, maternal chromosomes undergo elimination, resulting in haploids
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Arabidopsis
|
| 650 |
|
4 |
|a CENH3
|
| 650 |
|
4 |
|a GFP degradation
|
| 650 |
|
4 |
|a deGradFP
|
| 650 |
|
4 |
|a haploid induction
|
| 650 |
|
4 |
|a nanobody
|
| 650 |
|
7 |
|a Ubiquitin
|2 NLM
|
| 650 |
|
7 |
|a Proteasome Endopeptidase Complex
|2 NLM
|
| 650 |
|
7 |
|a EC 3.4.25.1
|2 NLM
|
| 700 |
1 |
|
|a Lermontova, Inna
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Moebes, Michael
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Kochevenko, Andriy
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Fuchs, Jörg
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Weiss, Oda
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Rutten, Twan
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Sorge, Eberhard
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Zuljan, Erika
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Giehl, Ricardo Fabiano Hettwer
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Mascher, Martin
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Somasundaram, Saravanakumar
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Conrad, Udo
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Houben, Andreas
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 73(2022), 22 vom: 08. Dez., Seite 7243-7254
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
|
| 773 |
1 |
8 |
|g volume:73
|g year:2022
|g number:22
|g day:08
|g month:12
|g pages:7243-7254
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1093/jxb/erac359
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 73
|j 2022
|e 22
|b 08
|c 12
|h 7243-7254
|