qHD5 encodes an AP2 factor that suppresses rice heading by down-regulating Ehd2 expression

qHD5 encodes an AP2 factor that suppresses rice heading by down-regulating Ehd2 expression

Copyright © 2022 Elsevier B.V. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant science : an international journal of experimental plant biology. - 1985. - 324(2022) vom: 01. Nov., Seite 111446
1. Verfasser: Bin Sun (VerfasserIn)
Weitere Verfasser: Xue, Pao, Wen, Xiao-Xia, Gong, Ke, Wang, Bei-Fang, Xu, Peng, Lin, Ze-Chuan, Peng, Ze-Qun, Fu, Jun-Lin, Yu, Ping, Sun, Lian-Ping, Zhang, Ying-Xin, Cao, Li-Ming, Cao, Li-Yong, Cheng, Shi-Hua, Wu, Wei-Xun, Zhan, Xiao-Deng
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2022
Zugriff auf das übergeordnete Werk:Plant science : an international journal of experimental plant biology
Schlagworte:Journal Article AP2 transcription factor Adaptation Heading date Rice qHD5 Transcription Factors
Beschreibung
Zusammenfassung:Copyright © 2022 Elsevier B.V. All rights reserved.
Heading date is crucial for rice reproduction and the geographical expansion of cultivation. We fine-mapped qHD5 and identified LOC_Os05g03040, a gene that encodes an AP2 transcription factor, as the candidate gene of qHD5 in our previous study. In this article, using two near-isogenic lines NIL(BG1) and NIL(XLJ), which were derived from the progeny of the cross between BigGrain1 (BG1) and Xiaolijing (XLJ), we verified that LOC_Os05g03040 represses heading date in rice through genetic complementation and CRISPR/Cas9 gene-editing experiments. Complementary results showed that qHD5 is a semi-dominant gene and that the qHD5XLJ and qHD5BG1 alleles are both functional. The homozygous mutant line generated from knocking out qHD5XLJ in NIL(XLJ) headed earlier than NIL(XLJ) under both short-day and long-day conditions. In addition, the homozygous mutant line of qHD5BG1 in NIL(BG1) also headed slightly earlier than NIL(BG1). All of these results show that qHD5 represses the heading date in rice. Transient expression showed that the qHD5 protein localizes to the nucleus. Transactivation activity assays showed that the C-terminus is the critical site that affects self-activation in qHD5XLJ. qRT-PCR analysis revealed that qHD5 represses flowering by down-regulating Ehd2. qHD5 may have been selected during indica rice domestication
Beschreibung:Date Completed 28.09.2022
Date Revised 28.09.2022
published: Print-Electronic
Citation Status MEDLINE
ISSN:1873-2259
DOI:10.1016/j.plantsci.2022.111446