Establishment of loop-mediated isothermal amplification for Brucella detection using a warmer pad as a heating source

The study sought to establish a sensitive and specific on-site loop-mediated isothermal amplification (LAMP) for Brucella heated using a warmer pad. LAMP primers specific to the conserved BvrR gene were designed, and the LAMP reaction was optimized. The heating characteristics of the warmer pad were...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1988. - 73(2022), 3 vom: 13. Sept., Seite 142-150
1. Verfasser: Yin-Bo, Ye (VerfasserIn)
Weitere Verfasser: Dong-Liang, Li, Bo-Han, Yang, Zhao, Zhang, Si-Yao, Mei, Bao-Shan, Liu, Ze-Liang, Chen
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2022
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Brucella BvrR LAMP brucellosis conducive control detection heating mehr... on-site warmer pad