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231226s2022 xx |||||o 00| ||eng c |
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|a 10.1107/S1600576722005891
|2 doi
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|a pubmed25n1149.xml
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|a eng
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|a Patel, Jaydeep
|e verfasserin
|4 aut
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|a Towards real-time analysis of liquid jet alignment in serial femtosecond crystallography
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|c 2022
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|a Text
|b txt
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Revised 02.09.2024
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|a published: Electronic-eCollection
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|a Citation Status PubMed-not-MEDLINE
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|a © Jaydeep Patel et al. 2022.
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|a Liquid sample delivery systems are used extensively for serial femtosecond crystallography at X-ray free-electron lasers (XFELs). However, misalignment of the liquid jet and the XFEL beam leads to the X-rays either partially or completely missing the sample, resulting in sample wastage and a loss of experiment time. Implemented here is an algorithm to analyse optical images using machine vision to determine whether there is overlap of the X-ray beam and liquid jet. The long-term goal is to use the output from this algorithm to implement an automated feedback mechanism to maintain constant alignment of the X-ray beam and liquid jet. The key elements of this jet alignment algorithm are discussed and its performance is characterized by comparing the results with a manual analysis of the optical image data. The success rate of the algorithm for correctly identifying hits is quantified via a similarity metric, the Dice coefficient. In total four different nozzle designs were used in this study, yielding an overall Dice coefficient of 0.98
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|a Journal Article
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|a automation
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|a image processing
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|a liquid jet alignment
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|a machine vision
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|a Round, Adam
|e verfasserin
|4 aut
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|a Bielecki, Johan
|e verfasserin
|4 aut
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|a Doerner, Katerina
|e verfasserin
|4 aut
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|a Kirkwood, Henry
|e verfasserin
|4 aut
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|a Letrun, Romain
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Schulz, Joachim
|e verfasserin
|4 aut
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|a Sikorski, Marcin
|e verfasserin
|4 aut
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|a Vakili, Mohammad
|e verfasserin
|4 aut
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|a de Wijn, Raphael
|e verfasserin
|4 aut
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|a Peele, Andrew
|e verfasserin
|4 aut
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|a Mancuso, Adrian P
|e verfasserin
|4 aut
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|a Ab Bey, Brian
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of applied crystallography
|d 1998
|g 55(2022), Pt 4 vom: 01. Aug., Seite 944-952
|w (DE-627)NLM098121561
|x 0021-8898
|7 nnas
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| 773 |
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|g volume:55
|g year:2022
|g number:Pt 4
|g day:01
|g month:08
|g pages:944-952
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|u http://dx.doi.org/10.1107/S1600576722005891
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