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231226s2022 xx |||||o 00| ||eng c |
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|a 10.1016/j.plantsci.2022.111414
|2 doi
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|a pubmed25n1149.xml
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|a (DE-627)NLM344822311
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|a (NLM)35963495
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|a (PII)S0168-9452(22)00239-4
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Zhao, Meiwei
|e verfasserin
|4 aut
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| 245 |
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|a The regulation of Alternaria alternata resistance by LRR-RK4 through ERF109, defensin19 and phytoalexin scopoletin in Nicotiana attenuata
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 01.09.2022
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|a Date Revised 13.12.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2022 Elsevier B.V. All rights reserved.
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|a Leucine-rich repeat receptor-like kinases (LRR-RKs), belonging to the largest subfamily of transmembrane receptor-like kinases in plants, are proposed to be involved in pathogen resistance. However, it is currently unknown whether LRR-RKs regulate Nicotiana attenuata resistance to Alternaria alternata, a notorious fungal pathogen causing tobacco brown disease. During transcriptome analysis, we identified a highly induced receptor kinase (NaLRR-RK4) in N. attenuata leaves after A. alternata inoculation. We speculated that this NaLRR-RK4 might be the resistance gene of tobacco to brown spot disease, and if so, what is its function and mechanism of action? Silencing of NaLRR-RK4 via virus-induced gene silencing (VIGS) lead to plants highly susceptible to A. alternata, and this result was further confirmed by two stable transformation lines (NaLRR-RK4-RNAi lines) generated by RNA interference technology. The susceptible of NaLRR-RK4-RNAi lines to A. alternata was associated with reduced levels of phytoalexin scopoletin and its key synthesis gene NaF6'H1. Further transcriptome analysis of leaves of WT and NaLRR-RK4-RNAi line after A. alternata inoculation revealed that NaLRR-RK4 regulated NaERF109 and NaDEF19. Silencing NaERF109 or NaDEF19 by VIGS lead to plants more susceptible to A.alternata, demonstrating their role in pathogen resistance. Interestingly, A.alternata-induced expression of NaF6'H1 and NaDEF19 were dramatically reduced in NaERF109-silenced VIGS plants. Taken all together, we identified LRR-RK4 as the first Leucine-rich repeat receptor-like kinases involved in A.alternata resistance in tobacco species, by regulating NaERF109, and subsequently NaDEF19 and NaF6'H1
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|a Journal Article
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|a A.alternata
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|a Na LRR-RK4
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|a NaDEF19
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|a NaERF109
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|a Nicotiana attenuate
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|a Sesquiterpenes
|2 NLM
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|a Leucine
|2 NLM
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|a GMW67QNF9C
|2 NLM
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|a Scopoletin
|2 NLM
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|a KLF1HS0SXJ
|2 NLM
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|a Phytoalexins
|2 NLM
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|a Ma, Lan
|e verfasserin
|4 aut
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1 |
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|a Song, Na
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cheng, Junbin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhao, Zhengxiong
|e verfasserin
|4 aut
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| 700 |
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|a Wu, Jinsong
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 323(2022) vom: 28. Okt., Seite 111414
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnas
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| 773 |
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|g volume:323
|g year:2022
|g day:28
|g month:10
|g pages:111414
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|u http://dx.doi.org/10.1016/j.plantsci.2022.111414
|3 Volltext
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