|
|
|
|
| LEADER |
01000caa a22002652c 4500 |
| 001 |
NLM34482229X |
| 003 |
DE-627 |
| 005 |
20250303170442.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231226s2022 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1016/j.plantsci.2022.111413
|2 doi
|
| 028 |
5 |
2 |
|a pubmed25n1149.xml
|
| 035 |
|
|
|a (DE-627)NLM34482229X
|
| 035 |
|
|
|a (NLM)35963493
|
| 035 |
|
|
|a (PII)S0168-9452(22)00238-2
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Kapoor, Ritu
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Stress responsive OsHyPRP16 promoter driven early expression of resistance gene Pi54 potentiate the resistance against Magnaporthe oryzae in transgenic rice
|
| 264 |
|
1 |
|c 2022
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 28.09.2022
|
| 500 |
|
|
|a Date Revised 28.09.2022
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Copyright © 2022 Elsevier B.V. All rights reserved.
|
| 520 |
|
|
|a The rice Hybrid Proline Rich Protein (HyPRP) encoding gene, OsHyPRP16 expression exhibit early upregulation in response to Magnaporthe oryzae inoculation. Here, we functionally characterized the OsHyPRP16 promoter through deletion analysis in transgenic Arabidopsis using GUS (β-glucuronidase) reporter assay. The promoter fragments, sequentially deleted from the 5' end could induce differential GUS activity in response to stresses induced by different hormones and abiotic stress conditions. In addition, a strong GUS induction was observed in M. oryzae inoculated transgenic Arabidopsis. Based on the insilico and stress-inducibility of D1 promoter fragment against various phytohormones and rice blast fungus, and with no basal activity under control conditions, we rationally selected D1 promoter fragment to drive the expression of a major rice blast resistance gene; Pi54 in the genetic background of blast susceptible TP309 rice line. The D1 promoter fragment was able to induce the expression of Pi54 at immediate-early stages of M. oryzae infection in transgenic rice. The transgenic plants with Pi54 under the control of D1 promoter fragment displayed complete resistance against M. oryzae infection as compared to control plants. The present study suggests that the D1 fragment of OsHyPRP16 promoter is a valuable tool for breeding and development of rice lines with early-inducible and pathogen-responsive enhanced disease resistance
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a GUS
|
| 650 |
|
4 |
|a HyPRP
|
| 650 |
|
4 |
|a Phytohormones
|
| 650 |
|
4 |
|a Promoter
|
| 650 |
|
4 |
|a Resistance
|
| 650 |
|
4 |
|a Rice blast
|
| 650 |
|
4 |
|a Stress
|
| 650 |
|
7 |
|a Hormones
|2 NLM
|
| 650 |
|
7 |
|a Plant Growth Regulators
|2 NLM
|
| 650 |
|
7 |
|a Plant Proteins
|2 NLM
|
| 650 |
|
7 |
|a Proline
|2 NLM
|
| 650 |
|
7 |
|a 9DLQ4CIU6V
|2 NLM
|
| 650 |
|
7 |
|a Glucuronidase
|2 NLM
|
| 650 |
|
7 |
|a EC 3.2.1.31
|2 NLM
|
| 700 |
1 |
|
|a Kumar, Gulshan
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Pawar, Lata
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Salvi, Prafull
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Devanna, Basavantraya N
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Singh, Kashmir
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Sharma, Tilak Raj
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 324(2022) vom: 28. Nov., Seite 111413
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnas
|
| 773 |
1 |
8 |
|g volume:324
|g year:2022
|g day:28
|g month:11
|g pages:111413
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1016/j.plantsci.2022.111413
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 324
|j 2022
|b 28
|c 11
|h 111413
|