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231226s2022 xx |||||o 00| ||eng c |
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|a 10.1002/aah.10169
|2 doi
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|a pubmed25n1149.xml
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|a (DE-627)NLM344783324
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|a (NLM)35959541
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Sugiura, Hidehiro
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Phenotypic differences between Edwardsiella piscicida and Edwardsiella anguillarum isolates in Japan
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| 264 |
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1 |
|c 2022
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 28.12.2022
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|a Date Revised 06.01.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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| 520 |
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|a © 2022 American Fisheries Society.
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|a OBJECTIVE: Edwardsiella tarda has been regarded as the causative agent of edwardsiellosis in cultured marine and freshwater fish species in Japan. Our previous study genetically classified an E. tarda-like isolate from diseased Olive Flounder Paralichthys olivaceus as E. piscicida and that from diseased Red Seabream Pagrus major as E. anguillarum. This study aimed to understand the phenotypic differences between E. piscicida and E. anguillarum
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|a METHODS: Fourteen E. piscicida and seven E. anguillarum isolates were used in this study. The colonies of each isolate were grown on brain-heart infusion agar plates and then subjected to DNA extraction. The extracted DNA was amplified using PCR. carbohydrate fermentation of the isolates was examined using API 50 CH test kits. Moreover, the growth of the two species was examined in defined media. Also, free amino acids in Olive Flounder and Red Seabream sera were detected and quantified via high-performance liquid chromatography-mass spectrometry. Statistical differences in the concentrations of free amino acids were analyzed using Welch's t-tests
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|a RESULT: The API 50 CH test revealed that L-arabinose and D-mannitol were fermented by E. anguillarum isolates but not E. piscicida isolates. Furthermore, the growth of E. piscicida and E. anguillarum was reduced in the defined medium without methionine and iron sulfate. The growth of E. piscicida was reduced in the defined medium without phenylalanine, tyrosine, alanine, or nicotinic acid, whereas the growth of E. anguillarum was reduced in the defined medium without serine, cysteine, leucine, threonine, or isoleucine. Tyrosine and alanine were present in higher concentrations in the Olive Flounder serum, whereas threonine and isoleucine were present in higher concentrations in the Red Seabream serum, suggesting favorable growth conditions for E. piscicida and E. anguillarum
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|a CONCLUSION: This study characterizes a minimal defined medium that can be used for developing vaccines against E. piscicida and E. anguillarum
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|a Journal Article
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|a Edwardsiella anguillarum
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| 650 |
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|a Edwardsiella piscicida
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| 650 |
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4 |
|a Japan
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| 650 |
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4 |
|a Olive Flounder
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| 650 |
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4 |
|a Red Seabream
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| 650 |
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7 |
|a Isoleucine
|2 NLM
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| 650 |
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7 |
|a 04Y7590D77
|2 NLM
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| 650 |
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7 |
|a Alanine
|2 NLM
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| 650 |
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7 |
|a OF5P57N2ZX
|2 NLM
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| 700 |
1 |
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|a Fukunishi, Kosuke
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kawakami, Hidemasa
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Imajoh, Masayuki
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of aquatic animal health
|d 1998
|g 34(2022), 4 vom: 12. Dez., Seite 197-207
|w (DE-627)NLM098202006
|x 1548-8667
|7 nnas
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| 773 |
1 |
8 |
|g volume:34
|g year:2022
|g number:4
|g day:12
|g month:12
|g pages:197-207
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| 856 |
4 |
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|u http://dx.doi.org/10.1002/aah.10169
|3 Volltext
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| 951 |
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|a AR
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| 952 |
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|d 34
|j 2022
|e 4
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|c 12
|h 197-207
|