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231226s2022 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.2c01368
|2 doi
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|a pubmed24n1148.xml
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|a (DE-627)NLM34471120X
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|a (NLM)35952001
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Dubackic, Marija
|e verfasserin
|4 aut
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| 245 |
1 |
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|a α-Synuclein Interaction with Lipid Bilayer Discs
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 24.08.2022
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|a Date Revised 29.08.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a α-Synuclein (aSyn) is a 140 residue long protein present in presynaptic termini of nerve cells. The protein is associated with Parkinson's disease, in which case it has been found to self-assemble into long amyloid fibrils forming intracellular inclusions that are also rich in lipids. Furthermore, its synaptic function is proposed to involve interaction with lipid membranes, and hence, it is of interest to understand aSyn-lipid membrane interactions in detail. In this paper we report on the interaction of aSyn with model membranes in the form of lipid bilayer discs. Using a combination of cryogenic transmission electron microscopy and small-angle neutron scattering, we show that circular discs undergo a significant shape transition after the adsorption of aSyn. When aSyn self-assembles into fibrils, aSyn molecules desorb from the bilayer discs, allowing them to recover to their original shape. Interestingly, the desorption process has an all-or-none character, resulting in a binary coexistence of circular bilayer discs with no adsorbed aSyn and deformed bilayer discs having a maximum amount of adsorbed protein. The observed coexistence is consistent with the recent finding of cooperative aSyn adsorption to anionic lipid bilayers
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|a Journal Article
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|a Research Support, U.S. Gov't, Non-P.H.S.
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| 650 |
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Amyloid
|2 NLM
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| 650 |
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|a Lipid Bilayers
|2 NLM
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|a alpha-Synuclein
|2 NLM
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| 700 |
1 |
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|a Liu, Yun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kelley, Elizabeth G
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hetherington, Crispin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Haertlein, Michael
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Devos, Juliette M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Linse, Sara
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sparr, Emma
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Olsson, Ulf
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1999
|g 38(2022), 33 vom: 23. Aug., Seite 10216-10224
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:38
|g year:2022
|g number:33
|g day:23
|g month:08
|g pages:10216-10224
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|u http://dx.doi.org/10.1021/acs.langmuir.2c01368
|3 Volltext
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|d 38
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|h 10216-10224
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