PARP14 promotes the growth and glycolysis of acute myeloid leukemia cells by regulating HIF-1α expression

PARP14 promotes the growth and glycolysis of acute myeloid leukemia cells by regulating HIF-1α expression

Copyright © 2022 Elsevier Inc. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 242(2022) vom: 15. Sept., Seite 109094
1. Verfasser: Zhu, Ying (VerfasserIn)
Weitere Verfasser: Liu, Zhirui, Wan, Yiqi, Zou, Liping, Liu, Liping, Ding, Shuangjin, Lu, Chen, Qiu, Fang
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2022
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Journal Article Acute myeloid leukemia Glycolysis HIF-1α NF-κB PARP14 Proliferation HIF1A protein, human Hypoxia-Inducible Factor 1, alpha Subunit Lactates mehr... NF-kappa B PARP14 protein, human EC 2.4.2.30 Poly(ADP-ribose) Polymerases Glucose IY9XDZ35W2
Beschreibung
Zusammenfassung:Copyright © 2022 Elsevier Inc. All rights reserved.
OBJECTIVE: Acute myeloid leukemia (AML) is an aggressive hematological malignancy with a poor prognosis. This study aimed to investigate the action of PARP14 in the growth and glycolysis of AML
METHODS: The clinical samples of AML patients were collected, and the expression of PARP14 was detected. AML cells were transfected with PARP14, HIF-1α or treated with NF-KB inhibitor (BAY11-7082) or PARP14 inhibitor (RBN012759). Cell proliferation was detected by CCK-8 and colony formation assays, apoptosis by flow cytometry, glucose consumption and lactate production by glucose and lactate kits, ECAR and OCR by XF96 bioenergy analyzer, and related protein levels by Western blot. A mouse xenograft tumor model was established to evaluate the effect of PARP14 on tumor formation
RESULTS: Significant upregulation of PARP14 expression was observed in AML. PARP14 promoted AML cell proliferation and glycolysis and inhibited apoptosis, while PARP14 deficiency had the opposite effect. PARP14 promoted HIF-1α expression by activating NF-κB. HIF-1α silencing reversed the cancer-promoting effect of PARP14. In vivo results suggested that PARP14 promoted tumor formation
CONCLUSION: PARP14 induces AML cell growth and glycolysis by activating NF-κB and promoting HIF-1α expression, which may suggest new insights into the pathogenesis of AML
Beschreibung:Date Completed 13.09.2022
Date Revised 11.11.2022
published: Print-Electronic
Citation Status MEDLINE
ISSN:1521-7035
DOI:10.1016/j.clim.2022.109094