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231226s2022 xx |||||o 00| ||eng c |
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|a 10.1002/adma.202203354
|2 doi
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|a pubmed24n1147.xml
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|a (NLM)35901787
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|a DE-627
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|e rakwb
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| 041 |
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|a eng
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| 100 |
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|a Latreille, Pierre-Luc
|e verfasserin
|4 aut
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| 245 |
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|a In Situ Characterization of the Protein Corona of Nanoparticles In Vitro and In Vivo
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 28.09.2022
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|a Date Revised 28.09.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2022 The Authors. Advanced Materials published by Wiley-VCH GmbH.
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|a A new theoretical framework that enables the use of differential dynamic microscopy (DDM) in fluorescence imaging mode to quantify in situ protein adsorption onto nanoparticles (NP) while simultaneously monitoring for NP aggregation is proposed. This methodology is used to elucidate the thermodynamic and kinetic properties of the protein corona (PC) in vitro and in vivo. The results show that protein adsorption triggers particle aggregation over a wide concentration range and that the formed aggregate structures can be quantified using the proposed methodology. Protein affinity for polystyrene (PS) NPs is observed to be dependent on particle concentration. For complex protein mixtures, this methodology identifies that the PC composition changes with the dilution of serum proteins, demonstrating a Vroman effect never quantitatively assessed in situ on NPs. Finally, DDM allows monitoring of the evolution of the PC in vivo. This results show that the PC composition evolves significantly over time in zebrafish larvae, confirming the inherently dynamic nature of the PC. The performance of the developed methodology allows to obtain quantitative insights into nano-bio interactions in a vast array of physiologically relevant conditions that will serve to further improve the design of nanomedicine
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|a Journal Article
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4 |
|a Vroman effect
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|a bio-nano interactions
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| 650 |
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4 |
|a differential dynamic microscopy
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|a in vivo quantification
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4 |
|a protein corona
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| 650 |
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|a Blood Proteins
|2 NLM
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| 650 |
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|a Polystyrenes
|2 NLM
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| 650 |
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|a Protein Corona
|2 NLM
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| 700 |
1 |
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|a Rabanel, Jean-Michel
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Le Goas, Marine
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Salimi, Sina
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Arlt, Jochen
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Patten, Shunmoogum A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ramassamy, Charles
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hildgen, Patrice
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Martinez, Vincent A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Banquy, Xavier
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Advanced materials (Deerfield Beach, Fla.)
|d 1998
|g 34(2022), 38 vom: 18. Sept., Seite e2203354
|w (DE-627)NLM098206397
|x 1521-4095
|7 nnns
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| 773 |
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|g volume:34
|g year:2022
|g number:38
|g day:18
|g month:09
|g pages:e2203354
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| 856 |
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|u http://dx.doi.org/10.1002/adma.202203354
|3 Volltext
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