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231226s2022 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2022.07.017
|2 doi
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|a pubmed25n1146.xml
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|a (DE-627)NLM343903539
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|a (NLM)35870442
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|a (PII)S0981-9428(22)00328-X
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Ruan, Mengjiao
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Cytosolic glucose-6-phosphate dehydrogenases play a pivotal role in Arabidopsis seed development
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 11.08.2022
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|a Date Revised 11.08.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2022 Elsevier Masson SAS. All rights reserved.
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|a Embryo development is essential for seed yield and post-germination growth. Glucose-6-phosphate dehydrogenase (G6PD), the rate-limiting enzyme in oxidative pentose phosphate pathway (OPPP), is widely involved in plant development and stress tolerance by providing nicotinamide adenine dinucleotide phosphate (NADPH). In this study, the double mutant (g6pd5/6), overexpression line (G6PD5/6OE) and complementation line (g6pd5/6Comp) of cytosolic glucose-6-phosphate dehydrogenases (Cyt-G6PD) were used to investigate Cyt-G6PD roles in embryo development of Arabidopsis. The results showed that the germination rate of g6pd5/6 seeds was delayed in comparison with that of Col-0; moreover, 11.5% of g6pd5/6 seeds did not germinate. The dysfunction of Cyt-G6PD resulted in decreased fresh weight and primary root length of g6pd5/6 seedlings. The height and silique length of g6pd5/6 plants were also decreased. Moreover, the abortion rate of siliques and seeds of g6pd5/6 plants were increased compared with those of Col-0, G6PD5/6OE and g6pd5/6Comp lines. However, the dysfunction of Cyt-G6PD did not affect pollen activity; but in g6pd5/6, the embryo development was partially delayed or inhibited. The contents of fatty acids and storage proteins, two main storage materials in Arabidopsis seeds, were decreased in g6pd5/6 seeds. Exogenous application of fatty acids (C18:2; C18:3) alleviated the delayed germination of g6pd5/6 seeds. RT-qPCR results further demonstrated that the early embryo development genes were down-regulated in g6pd5/6. Taken together, Cyt-G6PD plays a pivotal role in plant seed development by regulating the transcriptions of early embryo development genes and the accumulation of storage materials (especially fatty acids)
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|a Journal Article
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|a Arabidopsis
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|a Embryonic development
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|a Fatty acid
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|a G6PD
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|a Seed development
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| 650 |
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|a Arabidopsis Proteins
|2 NLM
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| 650 |
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|a Fatty Acids
|2 NLM
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| 650 |
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|a Glucosephosphate Dehydrogenase
|2 NLM
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| 650 |
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|a EC 1.1.1.49
|2 NLM
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| 700 |
1 |
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|a He, Wenliang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sun, Hao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cui, Chaiyan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Xiangxiang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Ruiling
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Xiaomin
|e verfasserin
|4 aut
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| 700 |
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|a Bi, Yurong
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 186(2022) vom: 01. Sept., Seite 207-219
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnas
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| 773 |
1 |
8 |
|g volume:186
|g year:2022
|g day:01
|g month:09
|g pages:207-219
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| 856 |
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|u http://dx.doi.org/10.1016/j.plaphy.2022.07.017
|3 Volltext
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|d 186
|j 2022
|b 01
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|h 207-219
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