Morphological and Molecular Characterization of Punctodera Stonei Brzeski, 1998 (Nematoda : Heteroderidae) from Virginia, USA

© 2022 Mihail R. Kantor et al., published by Sciendo.

Bibliographische Detailangaben
Veröffentlicht in:Journal of nematology. - 1969. - 54(2022), 1 vom: 16. Feb., Seite 20220008
1. Verfasser: Kantor, Mihail R (VerfasserIn)
Weitere Verfasser: Subbotin, Sergei A, Huse, Gregory, Handoo, Zafar A
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2022
Zugriff auf das übergeordnete Werk:Journal of nematology
Schlagworte:Journal Article Punctodera stonei United States Virginia cyst nematode detection diagnosis grass systematics taxonomy
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520 |a In August of 2021, several cysts with juveniles and eggs were discovered during a vegetation survey conducted at the Arlington National Cemetery, Virginia. Eight soil samples were collected from the rhizosphere region of the common grass (Festuca arundinacea L.) and processed at the Mycology and Nematology Genetic Diversity and Biology Laboratory (MNGDBL). Cysts were light to dark brown in color, and oval to pear-shaped without bullae in young cysts but present in older cysts and with prominent vulval cone. The juveniles had slightly concave stylet knobs projecting sometimes anteriorly, tail tapering gradually to a narrowly rounded terminus, and hyaline tail terminus conspicuous at least twice the length of stylet. The molecular analysis included the analysis of three gene sequence fragments: D2-D3 of 28S rRNA, ITS rRNA, and COI. The nematode species was identified by both morphological and molecular means as Stone's cyst nematode, Punctodera stonei. Detection of P. stonei in Virginia represents a new record of this species in the United States, and a second report after Canada in North America 
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700 1 |a Handoo, Zafar A  |e verfasserin  |4 aut 
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